Copyright Š 2010 – 2018 3DHISTECH LTD all rights reserved.

 

CONFIDENTIAL!

 

 

 

 

 

The following contents list contain all the links to the chapters about the housing, construction, mechanical, optical and electronics parts and components as well as adjustment procedures used and done in the scanners.

 

ˇ      Pannoramic Confocal, P250, SCAN, MIDI, DESK and 3DHISTECH iSaCS.

 

ˇ      Images with blue frame contain a link to the relevant chapter.

 

The following contents may be used as an alternative to the tree structure of the manual on Tablet and Android, if the browser does not display the navigation window correctly or otherwise correct links will not work. 

 

 

 

 

Select a chapter

 

Common articles for all scanners

 

3DHISTECH iSaCS

 

Pannoramic Confocal

 

Pannoramic 250

 

Common units S_M_D

 

Pannoramic SCAN

 

Pannoramic MIDI

 

Pannoramic DESK

 

 

 

 

 

 

 

         About

         Contents; summary (this file)

         What is new …

         Precautions

         Before replacing units

         Fault sheets

INI Files

Service program

Batch test program

Tutorials

How to …

Miscellaneous

User guides

Slide show; Video

 

 

 

 

 

 

 

 

 

 

 

 

 

Precautions; summary      PCON; P250; S_M_D

Mirrors and lenses

Electrostatic discharge

         Handling of extension cards and electronics devices

         FireWire and USB interface

         Connect the camera

Fluorescent light source

Autocollimator

Pannoramic SCAN

Pannoramic MIDI

Pannoramic DESK

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Tools and requirements; PCON; P250; S_M_D

Hardware tools

Software requirements  4

Slides for setup and checks  4

Tools used to cleaning optical surfaces  7

 

 

 

 

 

 

 

 

 

 

 

 

 

Slide scanner service          PCON; P250; S_M_D

 

General

Setup the program

Select USB Device

Option selector

Low Level Service

Common tools

USB Controller

General tools

Control of units

DC Controller tools

DC Motor control

Tools of P250

Tools of MIDI

Tools of DESK

 

Section [Microscope] of MicroscopeConfiguration.ini

 

SCAN with Objective changer

MIDI with Objective changer

 

 

 

 

 

 

 

 

 

 

 

 

          PCON; P250; S_M_D

… Adjust components and units

… Check …                                  

… Define …

… Exchange (spare) parts and units

… Install software and drivers

… Maintain scanners

… Upgrade units and components

… Use special techniques

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Cleaning optics  1;                         All scanners

General 1

Precautions to avoid cleaning of optical components  2

Warning! 2

 

Tools and materials used to cleaning optics  2

 

Decide, where the dust can be found  6

 

Cleaning techniques  8

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Step motor firmware upgrade; S_M_D

 

 

Requirements

Prearrangements

Start the Atmel programmer software

Post-processing

Install “Atmel AVR Tools” and “AVR Studio 4”

Programmer unit

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

FL filter block assembling         S_M_P250

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Addresses of scanner units

Unit

Address

Type

X-Y-Z control

00

C_P

USB-controller

01

All

DC-controller

02

P_S_M_D

X-motor

03

S_M_D

Y-motor

04

S_M_D

Z-motor

05

S_M_D

Turret unit

06

S_M_P

Tray loader motor

07

M_C

Slide loader motor

08

M_C

Objective changer

09

C_P_S_M

Camera changer

10

P

RGB illumination

11

C

Tilting unit

12

C

Immersion liquid unit

13

C

Mechanical shutter

14

C

Switch board

15

C_P

Legend: C=Confocal; P=P250; S=SCAN; M=MIDI; D=DESK

Define Address of unit              All scanners

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

FOV and Preview       All scanners

 

General

Theory

Creation of preview

Sample scan process

Relations and calculations

Preview camera’s view

PreviewAndBarcodeScanning

Field Of View

         Influence of the camera adapter

         Sensor characteristics

 

Pixel position and corrections

 

 

 

 

 

 

 

 

 

 

 

 

Fluorescent exciting   All FL scanners

 

General

Basics and components

            Visible light

            Fluorophore

            Photobleaching

            Filter block

            Characteristics of camera sensor

Principles of exciting and imaging

Define the components

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

All scanners

 

        

 

 

 

 

 

ˇ      All videos are created with a resolution of full HD 1080p (1920x1080pixels).

 

ˇ      If the speed of the internet connection is slow, the opened video may show an erratically (jerkily) flow (the video is not played continuously). Please load down it and play the video in the memory!

 

ˇ      A second reason might be the processor speed and available memory capacity because the video file will be decompressed before playing.

 

Please open the folder “Video” and select the file of interest!

 

 

 

 

 

 

 

 

 

                                                       

 

 

 

 

 

 

 

                   Overview

 

 

 

 

 

                   Housing

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Construction

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Slide view

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Slide Transporter

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Staining unit

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Wash - rinse

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Cover slipping

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                               Scanner; iSaCS

 

 

                   Construction

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Prerequisites

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Optics; illumination

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Preview

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Focus unit

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Objective changer

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   X-Y-Stage unit

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Tray unit; Slide handling

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Power; control

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Housing

 

 

 

 

 

 

 

Construction

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Prerequisites for Pannoramic scanners; PCON; P250; S_M_D

 

 

Introduction

Precautions

Computer configuration

Software and drivers

Hardware conditions and cabling

Preview camera

Scan cameras

Lumencor SPECTRA

Setup filters

Dongles

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Dongles                    PCON; P250; S_M_D

Barcode dongle

3DHISTECH dolgles

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Optics and illumination;          PCON

 

 

optical pathOverview

Brightfield illuminated optical path

Configure light sources and units

Components and construction

         Brightfield RGB illumination unit

         Condenser

         Slide Tissue Cover slip

         Immersion Liquid Feeder

         Objective

         Spinning disc unit

         Camera adapter

         Scan camera

Illumination path adjustments

Image path adjustment

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Preview;         PCON

 

Configure the preview unit

Preview components

Preview illumination unit

Areas of the slide

Mechanical adjustments

BF preview calibration

DF preview calibration

Barcode reading

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Focus unit

General

Functional overview of the focus unit

Components and construction

Focus unit

Condenser unit

Shutter mechanics

Adjustments; focus unit

Adjustments; condenser

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Objective changer; PCON

 

ˇ        Introduction

 

 

 

 

 

 

 

 

 

 

 

 

X- and Y-stage unit; PCON

 

General

            Enhancements in the PCON

Configure the X-Y-stage unit

Functional overview of the X-Y-Stage

X-Y-carriage system

Parallelogram

Specimen holder

Adjustment procedures

Check the hysteresis in Y-direction

X- and Y-carriage drive unit

            Dismount / mount the carriage drive unit

Exchange the X-Y-stage unit

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Tray and slide loader; PCON

 

General

Configure the tray unit

Components, construction

Setup and adjustment procedures

         Precautions

         General techniques

         Adjustment sequence

Test the adjustments

         Short test pass

         Tray- and slide loading test

Dismount and mount

         Remove the tray unit

         Mount the tray unit

 

 

 

 

 

 

 

 

 

 

 

 

Confocal unit

General

Configure

Basics and theory

Optical paths of the CC88

Components; construction

         FL filters

         Virtual tissue

Mountings and connections

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Power and control; PCON

General

Power input connector and main switch

Power supply

Housing (standby) Switch

Power distribution and switch board

USB Controller with EEPROM

Mechanical shutter

Immersion liquid feeder

RGB BF scan illumination

X-Y-Z-motor control

Stepper motors with control electronics mounted

Stepper motors without control electronics mounted

Temperature and fan control

Parts; Components

Cabling; summary

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                                              

 

 

 

 

 

 

 

 Housing  1

 

Stack cover 1

Stack cover mechanics  2

Upper cover 2

Upper cover lock  2

Upper cover mounting  3

Lower cover mounting  3

Remove the housing  4

Mount the housing  4

 

 

Construction  4

 

Adjust the truss position  6

 

Differences between P250BF and P250FL

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Prerequisites for Pannoramic scanners; PCON; P250; S_M_D

 

Introduction

Precautions

Computer configuration

Software and drivers

Hardware conditions and cabling

Preview camera

Scan cameras

Lumencor SPECTRA

Setup filters

Dongles

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Dongles                PCON; P250; S_M_D

Barcode dongle

3DHISTECH dolgles

 

 

 

 

 

 

 

 

 

 

 

Optics and illumination; P250

 

Overview

Components and construction

Flash light source

Neutral density (ND) filter unit

Collimator lens holder

 Condenser

Objective

Camera changer unit

Camera adapter

Brightfield scan (main) camera

Fluorescent scan camera

Image path adjustment

Check the optical path adjustments

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Focus unit with objective changer

 

General

Functional overview of the focus unit

Components and construction

Focusing part

Objective changer part

Shutter mechanics

Adjustments of the focus part and shutter mechanics

Adjustments of the objective changer part

Dismount or mount the focus unit with objective changer

Setup and define the implemented objectives

Align the Objective

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

X-Y-stage unit

 

General

         Enhancements in the P250

Configure X-Y-stage unit

Functional overview of X-Y-Stage

X-Y-carriage system

Adjustment procedures

Check the hysteresis in Y-direction

X- and Y-carriage drive unit

         Dismount őr mount Carriage drive unit

Exchange X-Y-stage unit

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Camera changer unit

 

General

Configure Camera changer unit

Functional overview of Camera changer unit

Components and construction of Camera changer unit

90º camera tube

Adjustment tools

Adjustment techniques

Adjust Camera changer unit

Dismount / mount Camera changer unit

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Preview unit

 

 Components of the preview unit

Horseshoe

Preview objective and camera holder

Preview camera and objective

Modified sensor position

 

Preview illumination unit

Scannable area

Adjustment procedures of the preview unit

Preview calibration procedure

Calibration program tools

Steps of the preview calibration procedure

Adjust the pixel of the preview camera to the field of view of Scan camera

 

Darkfield preview calibration procedure

Prerequisites for a successful darkfield preview calibration procedure

Calibrate the darkfield preview manually

Principle of the darkfield calibration

Execute the darkfield calibration

 

Barcode reading and adjustments

General

Adjust the barcode illumination

Barcode Settings dialog

Check barcode reading with different barcodes and types

Barcodes in practice

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 Magazine unit and slide handling; P250

 

General

 Enhancements in the magazine unit of the P250

Configure the magazine unit

Functional overview

Components and mechanical construction

         Magazine

         Software control

Input and output stack

         Magazine feeder channel

Remove or mount the magazine unit

Components and mechanical construction of Slide loader

Remove or mount Slide loader

Adjust the magazine load mechanics

Slide insert / removal (software)

Define and adjust Slide insert and removal positions

List of Parameters and explanation

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Power and control; P250

Power input connector and main switch

Power supply

Emergency power off (standby) switch

Power distribution & switch board

USB Controller with EEPROM

DC Controller

X-Y-Z-ND-motor and Flash light control

Stepper motors with control electronics

Stepper motors without control electronics

Temperature and fan control logic

Components

 

 

 

 

 

 

 

 

 

 

 

Reflector turret unit; belt

 

General

Configure the belt driven reflector turret unit

Functional overview of the reflector turret unit

Construction of Fluorescent illumination and image path

Construction

Mechanical components and construction

ˇ        Stepper motor and the belt wheel

ˇ        Filter wheel

ˇ        Mechanical drive

ˇ        Diaphragm position adjustment tools

ˇ        Iris size adjustment tools

ˇ        Mechanical shutter

ˇ        Remove / mount FL reflector turret unit

Optical components

ˇ        Light source adapter and mounting

ˇ        EPI-fluorescent illumination unit

ˇ        Mirror

ˇ        Filter block

ˇ        Virtual fluorescent tissue

Adjustment techniques / checks

ˇ        Tools, used for the adjustments

ˇ        Check / adjust the tightness of the belt

ˇ        Adjust the external sensor position

ˇ        Find the Home 1,2 position

ˇ        Find the first filter position

ˇ        Check correctness of the filter fixing in the filter positions

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                                                                 

 

 

 

 

 

 

Optics and illumination  1; S_M_D

 

Optics_Illumination.htmComponents and construction  4

Lamp housing  4

Lamp  4

Lamp tube  4

Illumination mirror; MIDI, DESK   5

Condenser 5

Objective  5

Camera tube  6

Camera tube mounting  6

Camera adapter 6

Scan (main) camera  7

 

Optics path adjustments  8

Illumination adjustment 8

Brightfield illumination adjustment parts  9

Adjust Uniformity of the illumination with Look up table  13

 

Image path adjustment 20

Chromatic aberration   20

Stitching  24

Adjust camera rotation angle  25

 

Check the optical path adjustments  27

Objective and focus position   27

Condenser position   28

Stripping of Scanned tissue  28

Y- and X-hysteresis  28

Chromatic aberration   29

Stitching  29

Stage skew check  29

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Camera changer (Double adapter 60N) 30

Adjustments  31

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Focus unit 2; S_M_D

 

Functional overview   4

Principle of focusing  5

Focusing algorithm   5

Scannable area  7

Define scan area  9

 

Components and mechanical construction  10

Shutter mechanics  12

 

Adjustment procedures  13

Adjust the ex-center position   13

Find the hardware limits for Focus unit 13

Mount the objective  14

Check or adjust the nominal focus positions  15

Adjust Condenser position   15

 

Dismount or mount Focus unit 17

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

X-Y-Stage unit 1; S_M_D

Functional overview of X-Y-Stage  2

X-Y-carriage system   4

Parallelogram   6

Specimen holders  6

Adjustment procedures  9

Check the maximal hysteresis in Y-direction   11

 

 

 

X- and Y-carriage drive unit 12

Spindle mounting  12

Transport spindle; Multi (4) thread spindle  13

Motor 13

Transport- and counter nut with spring  14

Dismount Carriage drive unit 14

Mount Carriage drive unit 14

 

Exchange X-Y-stage unit 16

 

 

 

 

 

 

 

 

 

 

 

 

 

 

USB and DC control;  S_M_D

 

 

Power supply

USB controller

addresses

         EEPROM

USB controller connections

DC-controller

 

Electrical components

Cabling; summary

 

 

 

 

 

 

 

 

 

 

 

 

 

Stepper motor

Stepper motor implementation

 

Construction of the stepper motor

 

Sensors Home1 and Home2

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Preview unit 2; S_M_D

Configure the preview unit

Preview components

Areas of the slide

Mechanical adjustments

BF preview calibration

Barcode reading

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Barcode reading and adjustments; S_M_D

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

System technique for panoramic scanners

ˇ      This chapter was replaced; please refer to

 

Prerequisites for Pannoramic scanners; PCON; P250; S_M_D

 

Precautions

Computer configuration

Software and drivers

Hardware conditions and cabling

Preview camera

Scan cameras

Lumencor SPECTRA

Setup filters

Dongles

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                                                                 

 

 

 

 

 

 

 Housing  1

Stack rail spacer 2

Camera protector 3

Side wall 4

Cover fixing  4

Shipping bolt 4

Stack covers  5

 

Move the SCAN  7

 

Shipping 7

Prepare SCAN and Cover for shipping  8

Pack the accessories  9

 

Construction  10

Base plate  11

Adjust the truss position  11

Truss  12

Scanner plate  13

Turret plate  15

 

 

 

 

 

 

 

 

 

Reflector turret unit; Gear

 

General

Functional overview

Construction of the fluorescent illumination and image path

Construction of the RTU

Mechanical components and construction

ˇ         Stepper motor

ˇ         Filter wheel

ˇ         Mechanical drive

ˇ         Diaphragm position adjustment tools

ˇ         Iris size adjustment tools

ˇ         Mechanical shutter

ˇ         Remove or mount the FL reflector turret unit

 

Optical components

ˇ         Light source adapter and mounting

ˇ         EPI-fluorescent illumination unit

ˇ         Mirror

ˇ         Filter block

ˇ         Virtual fluorescent tissue

 

Adjustments and checks

ˇ         Tools, used for the adjustments

ˇ         Check the correctness of the filter fixing in the filter positions

 

 

 

 

 

 

 

 

 

 

 

 

 

Magazine unit and slide handling 2

 

Functional overview of the Magazine unit 3

Components and mechanical construction of the magazine unit 5

Magazine load / unload 7

Magazine load procedure 9

Magazine unload procedure 9

Magazine feeder part 11

 

Adjust the magazine load mechanics 13

 

Components and mechanical construction of Slide loader 14

To move Slide loader moveable part manually 17

To adjust the inner finger vertical position 18

Check the function of Slide loader sensors 18

Slide loader parts for slide handling 18

Further slide handling parts 19

 

Slide insert / removal 20

Slide removal procedure 21

Define and adjust Slide insert and removal positions 21

Adjust the position of the magnet disc 23

Adjust the position of the magazine unit 23

Adjust Slide insert position in X-direction 24

Adjust the final insert position in Y-direction 25

Mistakes and abnormal / faulty behavior during slide insertion 26

Mistakes and abnormal / faulty behavior during slide removal 27

 

List of Parameters and explanation 28

 

Remove the magazine unit from Scanner 31

Mount the magazine unit 31

 

 

Please refer also to

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                                                                    

 

 

 

 

Housing  16

Open or remove shells  16

Mount shells  16

Remove the rear cover 16

 

Construction  17

Truss and base plate  17

 

 

 

 

Please refer also to

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 Tray- and slide loader unit 2

Functional overview of the tray- and slide loader unit 3

Tray transport unit 4

Slide loader and support plate mechanics  6

Setup and adjustment procedures  7

Precautions  7

General 7

The adjustments are done in the following sequence  8

Test the adjustments  15

Dismount / mount the tray- and slide loader unit 17

 

 

 

 

 

 

 

 

 

 

 

Reflector turret unit; Gear

 

General

Functional overview

Construction of the fluorescent illumination and image path

Construction of the RTU

Mechanical components and construction

ˇ         Stepper motor

ˇ         Filter wheel

ˇ         Mechanical drive

ˇ         Diaphragm position adjustment tools

ˇ         Iris size adjustment tools

ˇ         Mechanical shutter

ˇ         Remove or mount the FL reflector turret unit

 

Optical components

ˇ         Light source adapter and mounting

ˇ         EPI-fluorescent illumination unit

ˇ         Mirror

ˇ         Filter block

ˇ         Virtual fluorescent tissue

 

Adjustments and checks

ˇ         Tools, used for the adjustments

ˇ         Check the correctness of the filter fixing in the filter positions

 

 

 

 

 

 

 

 

 

 

 

 

 

                                                                 

 

 

 

 

 Housing  18

To remove Upper housing  18

To remove Lower housing  18

To mount Lower housing  18

To mount Upper housing  19

Slide door 19

 

 

 

Construction  19

Adjust Scanner plate position  21

Door closed position  22

To adjust the door sensor position  22

Adjust Specimen holder fixing  22

Position adjustment 22

Adjust the height 22

Adjust Supporter on Left side of Specimen holder 23

Check the gap between slide leader and Specimen holder 23

 

 

 

 

 

Please refer also to