Magazine unit, slide handling; SCAN

For technicians and partly for sales managers!

 

 

These instructions describe the procedures to install and adjust the magazine unit for Pannoramic SCAN scanners. To help resolve problems with the magazine unit or problems with slide insert or slide removal, a hardware description of the used components and adjustment procedures are added.

During the development of the Magazine unit of the SCAN, the components are more and more adapted to the components of the P250 in functionality, but mainly 3deviations existing:

  • The number of magazines, hold in the input and output stack is maximal 6 magazines, so the dimensions of the input and output stack are smaller.
  • The sensors Hall 1 and Hall 2 are not implemented, so the presence of magazines in the input and output stack is checked by the user.
  • The drive of the "Magazine load gear" is modified.

 

 

Contents

 

General

Functional overview

Configure the magazine unit

Components and construction

Slide loader; components, construction

Slide insert and removal (software)

Magazine load procedure (software)

Define, adjust slide insert and removal

Adjust the magazine load mechanics

Finishing adjustments

List of Parameters and explanation

Remove and mount the magazine unit

 

 

Important

If a software upgrade from the software version 1.14 to the software version 1.15 or higher is done, please adjust the position of the magnet disc again.

 

     Configure the magazine unit and “Magnet disc

         What is new …

 

For safety regulations regarding human health and scanner functionality please refer to:   Precautions

 

 

 

General

The magazine unit is a component added to the Pannoramic SCAN scanner to give the possibility of loading and removal of magazines and slides automatically. To handle the slides, magazines are used with 25 slide positions. The magazine input and output stack respectively can hold up to 6 magazines. The magazines are placed manually into the input stack and removed from the output stack by the user. The magazine load procedure transports the magazine with the help of the magazine load mechanism from the input stack to the magazine feeder.

 

The magazine feeder has 2 functions:

·    It transports the magazine from the magazine load position to the first slide position and from the last slide position to the magazine unload position.

·    It transports the magazine from slide position to slide position if slide change is done, after slide scanning is finished or to any desired slide position of the magazine.

 

The slide loader inserts the slide from the actual slide position of the magazine into the Specimen holder (slide holder) of the XY-Stage and after the scan procedure is finished it removes the slide from the specimen holder into the magazine. 

 

If automatic scan mode is selected all slides in the magazine are loaded sequentially from the magazine into the specimen holder, scanned by the scanner unit and returned into the magazine automatically after the scan process of the actual slide is finished. If all slides of the actual magazine are scanned the magazine is removed from the magazine feeder channel to the output stack meanwhile the next magazine will be loaded from the input stack, if there are any further magazines.

 

If manual scan mode is selected the Magazine load or unload and the slide load or unload procedures are controlled via software buttons. The mechanical movements are the same as described for automatic scan.

 

The exchange of the entire magazine unit is necessary

            - If one of the two magazine handling DC motors or the DC controller electronics is faulty.

            - If the shape of any part is deformed or a part is broken.

            - If the magazine unit has any fault and you are unable to fix it.

 

 

Requirements

  • Service program for Pannoramic scanners (SlideScannerService.exe)    with actual license file
  • Pannoramic SCAN and Pannoramic Viewer software (SlideScanner.exe, SlideViewer.exe) with actual license file
  • 1.5, 2.5, 3 and 6 mm hex wrenches,
  • Hardware and construction knowledge of Pannoramic SCAN

 

 

 

 

Attention     Do not mix the versions of SlideScanner.exe and SlideScannerService.exe! Always use these programs with the same version number. Otherwise the SlideScannerService.exe program could produce unwanted results and SlideScanner.exe does not work correctly or even freeze!

 

 

 

 

Functional overview

 

 Software control

 

  • In the program “SlideScanner.exe” the movement of the magazine is controlled via the magazine load and unload buttons in the tab “Routine work” if the manual scan operation is selected.

 

  • The slide loader inserts and removes the slide during the slide insert or removal operation.

 

·       To select a specific slide position of the magazine, the “Current slide” position may be incremented or decremented by the use of the buttons of the numerical field, or you may type a value between 1 and 25 into the numerical field.

·       By pressing the button “Slide insert” the magazine is moved to the desired slide position (shown in the numerical field) and then, the slide will be inserted. Is a slide in the specimen holder and the button “Slide insert” is pressed with a modified slide position, the slide will be removed first, before the magazine is moved.

 

 

 

 

 

 

 

 

 

 

 

Load a magazine

 

The “Magazine Loader” motor is started with the software button “Load Magazine”. By rotating the “Magazine Input Stack Wheels” the first magazine is placed onto the “Magazine Feeder Rails”. The end of the process is signaled by the “Magazine Loader Sensor” (not shown) and the “Magazine Loader” motor is switched off by software.

 

 

 

 

Unload a magazine

 

If the scan session of the actual magazine is finished or the magazine remove command is issued, the magazine is fed to the output stack position. The exact unload position is defined by the “Right magazine sensor” actuation again after the magazine passed it. Meanwhile the magazine is unloaded to the output stack the next magazine (if any exists) is loaded from the input stack to the feeder.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Feed a magazine

 

If a magazine arrived onto the magazine feeder rails the “Left Magazine Sensor” will be pressed. Even the weight of an empty magazine is able to push this sensor. This sensor signals the software, that a magazine had been loaded and a magazine is present in the magazine feeder channel. The magazine feeder motor is switched on by software and transports the magazine with the help of the feeder gear to the first slide position. During magazine feeding the “Magazine feeder sensor” (not shown) gives a pulse after each full turn of the feeder spindle to the software via the DC electronics and the USB electronics box. The software counts the pulses of the “Magazine feeder sensor” and finds the magazine position for the actual slide to be inserted. The distance between two slide positions is exactly 2 full turns of the feeder spindle.

 

       Magazine feeder unit

 

 

 

 

 

 

The slide loader inserts the slide to be scanned from the actual magazine’s slide position into the slide holder of the X-Y-Stage by pressing the “slide insert button” and removes the slide from the specimen holder and returns it to the slide position of the magazine, if a slide removal procedure is initiated by the “Slide remove” button. 

 

 

 

 

 

 

 

 

 

 

 

 

 

Newer version of the slide loader

 

     Slide loader

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

DC controller

 

The DC Electronics box is mounted onto the magazine unit from beneath. The commands to switch on or off the motors or the preview illumination are issued from the software (SlideScanner.exe or SlideScannerService.exe) via the USB Controller and the cable DCF-1. The status information (the state of the sensors) is read by the software on the same way. To prevent the motors from overload if hardware jamming occurs, all commands starting the DC motors have a time out. Normally, the motor is stopped with the sensor action before the time is run out. If the sensor action is missed (jamming occurred) the time out event will switch off the motor.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Configure the magazine unit

 

 

In the software version 1.15 or higher the components of the scanner SCAN 150 can be defined in the section [Microscope].

Because modifications are taken on the magazine unit and the slide loader; and these modifications have to be handled by the software also, a new parameter with value was implemented.

There are two parameter values possible:

 

LoaderType = SL_6Mag_25Slide_NoSensor_Vertical;          The magnet disc stops with the falling edge of the sensor

LoaderType = SL_6Mag_25Slide_NoSensor_Vertical_2;     The magnet disc stops with the rising edge of the sensor

 

The improvement includes:

  • The slide leader of the slide loader moveable part was removed.
  • The slide leading spring was mounted onto the magazine channel inner side wall.
  • The stop position of the magnet disc is found with the raising edge of the sensor. Please take this into account if you are working with the service program!

 

These modifications improve the stability of the magazine load procedure and the stability of the slide load procedure.

 

If the value is “SL_6Mag_25Slide_NoSensor_Vertical” or the parameter is missed in the file MicroscopeConfiguration.ini section [Microscope], the magazine load and unload operation and the slide insert or removal operation is done in traditional form, as done until the software version 1.14.

 

  • This solution has to be used if the slide leader is present on the slide loader’s moveable part!

 

LoaderType = SL_6Mag_25Slide_NoSensor_Vertical

 

This type uses the “falling” edge of the magazine feeder sensor!

 

 

 

 

 

 

 

Slide leading spring

 

If the value is “SL_6Mag_25Slide_NoSensor_Vertical_2” in the file MicroscopeConfiguration.ini section [Microscope], the magazine load and unload operation and the slide insert and removal operation is modified. The new magazine unit type can be easily recognized by the presence of the slide leading spring on the inner side wall and the slide leader of the slide loader’s moveable part is missed.

 

  • This “LoaderType” should be used if the slide leader is not present on the slide loader’s moveable part and the slide leading spring is present!

 

LoaderType = SL_6Mag_25Slide_NoSensor_Vertical_2

 

This type uses the “raising” edge of the magazine feeder sensor!

 

 

 

 

 

 

Important

If the value of the parameter “LoaderType” was changed in the file MicroscopeConfiguration.ini section [Microscope], the magazine load and unload procedure and the slide insert and removal procedure has to be adjusted again (readjust the position of the magnet disc)!

 

·       If an upgrade of the magazine unit was done, please implement the parameter “LoaderType” into the section [Microscope].

 

 

 

 

 

 

Before adjustments are done with the service program, the appropriate edge of the magazine feeder sensor has to be selected or checked.

 

If the value of the parameter is:

 

LoaderType = SL_6Mag_25Slide_NoSensor_Vertical

 

Or no parameter exists for the loader type (software versions, before the version 1.15) Select the “falling” edge and

 

If the value of the parameter is:

 

LoaderType = SL_6Mag_25Slide_NoSensor_Vertical_2

 

Select the “raising” edge!

 

 

 

 

 

 

 

          Parameter options of the section

 

 

 

 

 

 

 

Components and construction

 

 

1           Magazine unit mounting

2a         Magazine input (left) sensor

2b         Magazine output (right) sensor

3a, 3b   Slide loader mounting

4           Slide leading spring with holder

5           Magazine feeder motor

6           Magazine feeder drive

7           Magazine feeder wheels

8           Magazine supporter

9           Magazine stack wheels

 

 

 

 

 

                   Magazine unit mounting

 

The entire magazine unit is mounted to the truss by the use of 4 washers and bolts (1).

 

·       If the magazine unit should be dismounted, please remove the bolts.

·       If the position of the magazine unit should be adjusted, please loosen the bolts barely.

 

          Truss”; “Magazine unit position"

 

 

 

 

 

 

 

 

                   Dismount magazine unit

1.      Remove the preview illumination cable BGF-1.

 

2.      Remove the cable HMF-1 from the slide loader and remove the entire slide loader also.

 

3.      Remove the cable DCF-1 from the DC controller unit.

 

4.      To remove the "Unit position adjustment cover", remove and loosen respectively the bolts as shown; the cover can be moved downward. Loosen the adjustment fixing bolt a little bit.

 

5.      Remove the mounting bolts for the Magazine unit (1) see above.

 

6.      Move the entire magazine unit upward.

 

 

 

 

 

 

 

 

 

 

               Mount magazine unit

1.      Place the magazine unit onto the truss.

 

2.      Insert the mounting bolts with the washers and tighten these carefully.

 

3.      Connect the cable DCF-1 for the DC controller unit.

 

4.      Connect the preview illumination cable BGF-1.

 

5.      Insert the slide loader and connect the cable HMF-1.

 

6.      Adjust the magazine loader as described in “Adjust the magazine load mechanics”.

 

7.      Adjust or check the magazine unit position as described in “Define the slide insert and removal positions”.

8.      Mount the cover of the magazine unit position adjustment.

 

9.      Execute the magazine load and unload procedures with the service program.

 

10.  Execute the slide loading test of the service program with different slide sizes, slide shapes and different magazines also.

 

 

 

                   Magazine unit position

 

It may happen that the magazine unit needs to be removed for some adjustments. if the position of the magazine unit is already adjusted, and the unit needs to be removed, the adjusted position would be lost.

With the adjustable bumper (2) the magazine unit position is defined.

If the magazine unit is placed onto its mounting, easily push the entire unit against the bumper, tighten the unit position fixing (3) barely and tighten the Magazine unit mounting bolts from the top.

 

If the magazine unit position should be adjusted, the magazine unit mounting bolts (1) must be loosened a little bit to make the magazine unit barely moveable. With the bumper position (2) adjustment and the position fixing (3) bolt the entire magazine unit can be moved to the left or to the right to find the correct position for the slide position guide and the inner finger of the slide loader in relation to the specimen holder.

The position fixing bolt should be loosened if the position adjustment bolt is turned clockwise; the entire magazine unit moves to the left.

 

Important

If the unit position bumper is turned counter clockwise, please pull the entire Magazine unit manually to the right, until the bumper is reached again!

 

 

 Adjust the magazine unit position

If the position adjustment bolt is turned counter clockwise (loosened), the entire magazine unit can be moved to the right by carefully tightening the position fixing bolt.

If the adjustment is finished, the position adjustment bolt should touch the magazine unit and the position fixing bolt should be even tighten. With this solution the adjusted position is not lost and the correct position of the magazine unit is found again, even if the entire magazine unit was removed.

 

          Fixing fork; Check and adjust the magazine position; Adjust the position of the magazine unit; Adjust the position of the specimen holder fixing fork

 

 

 

                   Magazine

 

The magazine has 25 positions and can so contain up to 25 slides with a thickness of up to 1.4 mm, meaning the thickness of the cover slip and the barcode label is included. Insert the slide into the magazine so, that the cover slip shows always to the objective during slide insertion or scanning. The slide position numbering is realized on the upper edge of the rear wall. 

The magazine is moved with the feeder gears and the rack on the rear of the magazine.

A metal plate (to increase the slide removal security) is integrated.

 

  • The slide positions of the magazine should be kept clean and dry.
  • The magazine does not need adjustment.

 

Remark

The permanent magnet is used in the P250 only. Every magazine with 25 slide positions contains a permanent magnet to reduce the amount of different magazines.

 

 

 

 

 

 

 

 

 

 

 

                   Slide dimensions

 

 

Allowed slide dimensions are

Length:          75.00 to 76.00 mm

Width:            25.00 to 26.00 mm

Thickness:    00.95 to 01.05 mm

 

Since January 2015 a slide dimension’s check tool is delivered with the scanner.

 

  • If the first character of the serial number is an S the tool is used to check the slide dimensions of the scanners “SCAN, “MIDI” and “DESK””.

 

  • If the first character of the serial number is a P the tool is used to check the slide dimensions of the scanner “P250”.

 

 

·      Please check the slide dimensions before filling the magazine!

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                      Slides

 

The cover slip should be in distance from the lower slide edge, in distance from the slide holding mechanics of the specimen holder; not less than 1.0mm from the slide edge.

Because the slide holds the tissue to be scanned, the slides have to fulfill optical requirements also.

 

The slide bottom (the surface, oriented to the condenser) must be clean and dry.

  • Dust and paraffin residues may result in focusing difficulties (because the focus pin contacts this surface) or may generate vibration during the slide scan process, resulting in reduced quality of the virtual tissue.
  • The slide is mechanically fixed by the slide clamp on the barcode area. Therefore, never use stickers or labels on the bottom of the barcode area, because these produce a deviation of the field of view in relation to the optical axis; the result will be a “Slanted FOV”.

 

          The stage skew check”.

 

 

The top of the slide (the surface, oriented to the objective) holds the tissue together with the cover slip.

  • Keep the top of the cover slip clean and dry. Dust or paraffin residues may cause focusing difficulties during the slide scan process, resulting in reduced quality of the virtual tissue.
  • Before inserting the slide into the magazine, wait, until the cover slip glue is already dry, otherwise, residues may occur on the slide slot bottom of the magazine and then slide loading difficulties are the result.

 

          Specimen holder”; “Cleaning optics

 

 

 

 

                   Magazine fixing pins

 

 The magazine fixing pins fix the position of the magazine during slide insertion and slide removal.

 

  • They need no adjustment or maintenance.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Optical sensor

 

If the optical sensor OPF-1 and 2 (left and right magazine sensor) is pushed by the magazine, the light path between LED and photo transistor will be broken by a pin. This action is recognized by software.

 

The sensor positions are fixed and they are situated in the magazine feeder channel bottom plate. The sensors do not need adjustment.

 

  • The sensors should be kept clean. Because the action path is very short, even small glass shards or dust is able to prevent the sensor from correct switching.

 

 

 

 

 

 

 

 

 

 

 

 

Magazine input (left) sensor

 

 This sensor is used to detect a magazine, after a magazine load procedure is finished. Even an empty magazine pushes this sensor. There are two possible events. Both can occur and are fully equivalent in function of the magazine load procedure, but the first version should be preferred. The second version is a compromise, because the magazine feeder magnet disc has two tasks but offers only one adjustment possibility; the compromise was done for the magazine input sensor.

 

 

1.     The magazine arrives onto the feeder rail and the sensor is pressed

In this case, the software starts the magazine feeder gear one turn backward (the magazine is moved to the left) and checks the state of the Magazine input sensor again. The sensor should be released. If the sensor is released, one turn forward (the magazine is moved to the right) of the feeder gear follows and the sensor should be pressed again. This way, the software checks the function of the sensor and the presence of the magazine. This behavior is preferred and has higher priority than the second version, described in the following.

 

2.     The magazine arrives onto the feeder rail and the sensor is not pressed

If the sensor is not pressed during the magazine loading (the magazine loader sensor HAC-1 is sensed), the feeder gear is turned forward by one turn (the magazine is moved to the right) and the sensor state is checked again.

If the sensor is now pressed, the software detected the presence of the magazine.

If the sensor is not pressed, the software detected “No magazine present” and stops magazine load and feed actions and the message “Magazine not loaded” is displayed.

 

 

Magazine output (right) sensor

 

This sensor is used to detect the correct unload position for the magazine. The sensor is pushed and will be released if the magazine is moved to the right (forward). If the software recognized, that the sensor is released, a turn backward follows (the magazine is moved to the left). If now the sensor is pressed again, the right magazine unload position is found and the unload procedure is started, otherwise the software recognizes “magazine jamming occurred” if the time out of the feeder gear is run out.

 

 

                   Magazine loader unit

 

 

The magazine loader unit consists of:

·      Magazine loader motor

·      Magazine load gear

·      Magazine load drive

·      Input and output stack wheels

·      Magazine load sensor

 

The magazine load and unload drive part of the magazine unit moves the magazines in vertical direction, from the magazine input stack onto the magazine feeder rails and from the magazine feeder rails to the output stack respectively by the help of the magazine load wheels. Both actions are realized with the same motor and mechanical drive, so they are done always at the same time. Meanwhile the actual (finished) magazine is moved upward to the output stack, the next magazine (with slides to be loaded) is moved downward from the input stack to the magazine feeder channel. This combined mechanical movement is always done, even if the first magazine is being loaded or the last magazine will be unloaded.

 

 

 

 

The magazine loader motor is a 24V DC motor and is switched on via software with the button “Load Magazine”. In the service program, this motor can be driven in forward and backward direction respectively.

The backward direction can be used for test purposes only without a magazine in the input stack. The software uses the forward direction only, without exception.

To increase the torque of the motor, a gear is connected directly.

 

·       The “Magazine Loader Motor” does not need maintenance or adjustments.

 

 

       DC controller

 

 

 

 

 

 

 

      Magazine load gear

 

 The load gear drives the worm spindles, both in the same direction. If magazine jamming occurred, the gear can be driven manually on the motor axle.

·       The “Magazine Load Gear” does not need maintenance or adjustments.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

  

      Load drive and stack wheels

 

The input and output stack wheels are situated on both sidewalls of the magazine feeder channel and are driven by the worm spindle so, that the output stack wheels moving the magazine upward during the input stack wheels lowering down the magazine. To reach the opposite movement of the stack wheels, the drive parts “A” and “D” of the worm spindle are mounted in opposite direction in relation to the drive parts “B” and “C”. With this solution, the required movement of the stack wheels can be reached.

 

  • The Stack wheels do not need maintenance.
  • By adjusting the magazine load sensor position, the correct stop position of the stack wheels can be found.

 

 

 

 

 

 

 

 

      Hall sensor

 

The “Magazine Loader Sensor” and the “Magazine Feeder Sensor” are realized with Hall elements. If the south pole of a magnet is over the hall element the switch is closed and this state is recognized by the software.

If the other polarity of the magnet is over the hall element no action occurs.

 

  • The sensor position is adjustable. For adjustment procedures see the appropriate chapters.
  • The sensor does not need maintenance.

 

       Hall sensor

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Magazine load sensor

 

The magazine input stack wheel, situated behind the sensor HAF-1, contains two permanent magnets in a distance of exact 180 degrees in relation to each other.

The sensor senses so a half revolution of the “Input and Output stack wheels”.

After loosening the sensor’s mounting bolt, the sensor can be moved upward or downward respectively. The right sensor position is found if the stack wheels are in vertical position and the sensor is attached.

 

  • The sensor does not need maintenance.

 

       Hall sensor

 

 

 

 

 

 

 

 

 

                   Magazine feeder channel

 

The magazine feeder channel consists of the following main parts:

 

 

The inner side wall with:

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

The outer side wall with:

 

 

 

 

 

 

 

 

 

 

The bottom plate with:

 

 

 

  • The entire magazine feeder channel should be kept clean and dry.
  • Dust and glass shards can be removed easily with a brush from the magazine bottom plate and other parts.

 

 

 

 

 

 

 

 

 

 

                   Magazine supporter

 

On the inner and on the outer side wall of the magazine channel output stack part magazine supporters are realized.

The supporters are required, if magazines are present in the output stack and no magazine is in the magazine feeder channel during a magazine load procedure is in progress.

If the supporter would be missed (or it works wrong) the magazine would falling down onto the output stack wheels and magazine jamming might occur.

 

 

 

 

Solution

 

If the magazine moves upward from the feeder channel in direction to the output stack, the supporters are pushed outward by the front and the rear part of the magazine (1).

The diameter of the magazine output stack wheels is some 10th mm more than the height of the support surface (2); so, if the vertical position of the magazine output stack wheels is reached, the spring pushes the support surface (2) under the magazine.

If now a magazine unload procedure is in progress without a magazine in the feeder channel, the magazine(s) moving downward only some 10th mm and then the magazine movement is stopped by the support surface (2).

 

 

Remark

The supporter exists in newer versions only!

 

 

 

 

 

 

                   Input and output stack

 

The stack contains the magazines for slide scanning in the input stack and holds the finished magazines in the output stack. In the input stack as well as in the output stack maximal 6 magazines can be placed.

 

Stack rails

The stack rails are rotated by 30 degrees against the plump vertical (as well as the entire magazine unit) and so the slides are placed always on the rear of the magazine by gravity. This solution ensures also a smooth downward and upward movement of the magazine during the load and unload procedure.

 

Stack rail mounting

The stack rails are mounted to the outer magazine channel side wall by 3 bolts for each rail; on the other end, the stack rails are glued into the stack rail spacer.

 

Stack rail spacer

It defines the upper distance of the stack rails to each other and guarantees the parallelism of the stack rails.

 

Important

The stability of the stack rail spacer is not designed for any movements or the transport of the scanner; it is not a handle!

 

 

 

 

                   Magazine feeder part

 

The magazine feeder channel together with the feeder rails ensures proper movements of the magazine in horizontal direction, to the desired slide position or to the magazine unload position. If the magazine was just loaded, the magazine will be moved automatically (controlled by software) to the first slide position of the magazine.

 

 

  • The shape of the magazine feeder rail "D" is shown incorrect. The correct shape is shown in the chapter "Magazine feeder rails".

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Magazine feeder rails

The feeder rails are different in shape, because the magazine is driven only on the back side of the magazine by the magazine feeder gear. To ensure very small surface resistance during feeding on the non driven side of the magazine, “Rail 1” has a “D” shape. To lead the magazine during feeding and to ensure stable movement of the magazine, “Rail 2” has a square profile.

 

·       In some versions, the “D” shape may be altered to an “O” shape.

 

·       The rails should be kept clean and dry.

·       The magazine feeder rails do not need adjustment.

 

 

 

 

                   Magazine feeder gear mounting

 

The mounting bolt (A) together with the force of the spring guarantees the optimal connection between worm spindle and feeder wheels during feeding. This solution protects the motor also from overload during magazine feeding.

 

·       If magazine jamming occurs the spring allows a disconnection of the drive spindle from the feeder wheel. 

·       By disconnecting the drive unit from the feeder wheels manually (Pull), the mounting plate will be rotated frontward in the mountings (B and C) and so the magazine becomes manually moveable in the feeder channel (pull the mounting plate frontward).

 

·       The spring should not be stretched or shortened!

 

 

 

                   Pull the mounting plate frontward

 

During adjustments, checks and services, the feeder drive unit can be disconnected manually from the feeder wheels and so the magazine becomes manually moveable in the magazine feeder channel.

By pulling the mounting plate frontward, the compression spring of the mounting bolt will be pressed, the drive of the feeder wheels is disconnected and so:

  • The magazine can be shifted to any desired slide position manually.
  • The magazine can be removed from the magazine channel manually by shifting the magazine to the right and
  • The magazine can be inserted into the magazine feeder channel manually from the left or right side.

 

If the mounting plate is released again, the right connection between worm spindle and feeder wheels should be checked.

 

·       Rotate all three feeder wheels a little bit or

·       Issue the Magazine Feeder Forward command via the service program.

 

  • The “Magazine feeder gear” does not need maintenance.

 

 

 

                   Magazine feeder motor

 

The magazine feeder motor is a 24V DC motor and it is switched on via software to reach the desired slide position; the first slide position after magazine load or to reach the magazine’s unloading position. With the software (SlideScanner.exe or SlideScannerService.exe) the backward direction of this motor is used to reach previous slide positions also.

To increase the torque of the motor, a gear is connected directly. The transmission of this gear is different to the gear of the magazine loader motor.

 

·       The “Magazine feeder motor” does not need maintenance or adjustments.

 

       DC controller

 

 

                   Magazine feeder drive

   

The magazine feeder gear moves the magazine in the feeder channel via the worm spindle and the feeder wheels. Because the position of the feeder wheels during the magazine load action is different from the position during the slide load action, the magnet disc contains two permanent magnets; it is situated near the end of the worm spindle. In newer solutions, one revolution of the worm spindle moves the magazine a half slide position. To reach the next slide position, exactly two revolutions of the worm spindle is needed. The first magnet of the magnet disc  initiates a pulse via the sensor to the software after each revolution of the worm spindle. The pulse is used to switch off the motor. For slide insert actions always and only the forward stop position of the feeder gear is used, during the magazine load action is always done in the backward stop position. If previous slide positions should be reached, the worm spindle is turned backward one turn more as necessary, and then a forward turn of the feeder gear follows to fulfill this requirement. Please take this into account if you are adjusting the slide insert or removal position with the service program!! Before a magazine load procedure is done, the magnet disc should be in the start position, otherwise the cogs of the magazine rack and the cogs of the feeder wheels will not engage correctly.

 

 

 

 

 

 

 

 

 

                   Magazine feeder wheels

 

The feeder wheels are driven by the worm spindle from the outer side, during the inner side of the feeder wheel drives the magazine rack.

To prevent a mechanical shorting during magazine movements in the feeder channel if the magazine connects the middle feeder wheel or the feeder wheel on the right, the left and right sided feeder wheels are not fixed in their positions; these are fixed with springs, inside the wheel mounting.

The acting range of the left and right handed feeder wheels is about 0.5mm. The acting direction is always outward; it means, the left feeder wheel has the acting direction to the left and the right handed feeder wheel has its acting direction to the right.

To ensure a stable slide loading position of the magazine, the feeder wheel in the middle is fixed and has no acting range.

 

 

                   Feed a magazine

 

  • If magazine feeder jamming occurs often (if the magazine rack drive changes from the left to the middle feeder wheel or the drive changes from the middle to the right sided feeder wheel), check the acting range and the acting direction of the feeder wheels!
  • To check this, please remove the mounting bolt with spring, disconnect the feeder sensor and rotate the feeder unit mounting plate downward.

 

Important

  • If the acting direction of any feeder wheel is different or there is no acting range, a shorting of the mechanics of the feeder drive unit occurs (magazine jamming) and feeding of the magazine will never work correctly.

 

 

                   Magnet disc

 

The magnet disc, together with the Feeder sensor is used to stop the magazine movement in the feeder channel at defined positions; these positions depending on the SCAN version.

 

In MIRAX SCAN (magazines with 50 slide positions)

·       The magnet disc selects the previous or the next slide position of the magazine by exact 1 revolution; the sensor acts with the falling edge.

 

MIRAX SCAN upgraded (magazines with 25 slide positions)

·       The magnet disc selects the previous or the next slide position of the magazine by exact 2 revolutions; the sensor acts with the falling edge.

 

SCAN 150 (magazines with 25 slide positions)

·       The magnet disc selects the previous or the next slide position of the magazine by exact 2 revolutions; the sensor acts with the falling edge.

 

Pannoramic SCAN (magazines with 25 slide positions and Slide Leading Spring not present; no slide loader type or the LoaderType = SL_6Mag_25Slide_NoSensor_Vertical)

·       The magnet disc selects the previous or the next slide position of the magazine by exact 2 revolutions; the sensor acts with the falling edge.

 

Pannoramic SCAN (magazines with 25 slide positions and Slide Leading Spring; LoaderType = SL_6Mag_25Slide_NoSensor_Vertical_2)

·       The magnet disc selects the previous or the next slide position of the magazine by exact 2 revolutions; the sensor acts with the rising edge.

 

To define the scanner type in front of us, the following criteria can be used:

1.      Check the file “MicroscopeConfiguration.ini” section [Microscope] for the presence and the type of LoaderType =”

2.      Check the number of slide positions in the magazine

3.      Check the presence of the slide leader on the slide loader; if present, the sensor acts with the falling edge.

4.      Check the presence of the slide leading spring on the inner side wall; if present, the sensor acts with the rising edge.

 

To adjust the magnet disc position in relation to the spindle or the magazine position, the fixing must be barely loosened to rotate the disc on the spindle and tightened again before position checks are done.

 

The magnet disc position is in connection with two actions, the “slide insert and removal” procedure as well as the “magazine load and unload” procedure; both depending on the right adjustment:

 

     Configure the magazine unit” and “Magazine load procedure

 

The magnet disc’s stop position depends on the type of the magazine unit version.

·       Since the software version 1.15, the magazine unit type can be defined.

 

If there is no parameter of the loader type defined in the file MicroscopeConfiguration.ini section [Microscope] or the “LoaderType” is:

 

·       LoaderType = SL_6Mag_25Slide_NoSensor_Vertical;         the magnet disc stops with the falling edge of the sensor; the magnet disc stops on the magnet (sensor state in the service program:         ü= acting position found).

 

If the “LoaderType” is:

·       LoaderType = SL_6Mag_25Slide_NoSensor_Vertical_2;     the magnet disc stops with the rising edge of the sensor; the magnet disc stops behind the magnet (sensor state in the service program:            X = unattached or unknown).

 

·       If you are adjusting the magazine unit please set the appropriate “magazine feeder sensor edge” first, after starting the service program!

 

 

 

                   Set the feeder rising edge

1.      Start the service program “SlideScannerService.exe” and switch active the section “Low level service” and “DC controller”.

2.      In the tab „Advanced”, section “Magazine feeder” check or select the “Edge of sensor” to the option “raising” and close the dialogue “Advanced” with OK!

 

·       In the same way, the falling edge may be defined.

 

·       In opposite to the P250, in the Pannoramic SCAN or the SCAN 150 the raising edge or the falling edge may stop the magazine feeder motor; depending on the scanner version!

 

 

 

 

 

 

 

 

 

 

 

1.     The magazine load action

The position of the cogs of the magazine rack and the cogs of the feeder wheels should allow the cogs to match up to ensure, that the magazine moves downward smoothly and evenly. If the magazine arrived onto the feeder rails, the magazine input sensor should be pressed. The correct start position of the magnet disc for the magazine load procedure is found by rotating the magazine feeder gear backward (with the service program); see also (1) on the right.

 

The magnet disc stop position should be anywhere over the second magnet (depending on the “LoaderType”; see above and on the right) and the sensor should signal the active state (SlideScannerService.exe, DC controller, Sensor state, Magazine feeder= on).

 

2.     The slide insert and removal action

The correct slide insert or removal position is also found by adjusting the “Magnet disc” position. The slide loader outer finger should be in the middle of the magazine rail. The correct position of the magnet disc for the slide load procedure is found by rotating the magazine feeder gear forward (with the service program); see also (2) on the right.

 

 

 

 

 

The slide insert and removal action has higher priority then the magazine load action, during adjustments!

It is important that after each turn of the feeder spindle, the stop position of the disc should be always the same, if the option “Stop with sensor” is selected. To reach this requirement, the position of the sensor in relation to the magnet disc can be adjusted in a small range.

The detailed adjustment procedure is described later.

 

If the feeder gear has very easy motion, the stop position of the magnet disc is found beyond the second magnet. In these cases, the software initiates a backward movement of the feeder motor and so the sensor still gives a signal. Use this stop position (after the backward movement was done) to adjust the magazine’s slide rail in relation to the slide loader outer finger.

 

·       The magnet disc and the sensor do not need maintenance.

 

 

 

                   Sensor disc modified; since 2014

 

Working principle

By implementing 2 further permanent magnets into the existing magnet disc, the stability of the recognition of the magazine presence in the feeder channel is improved. While the slide insert position remains unchanged; the left magazine sensor will be surely attached if a magazine is present.

 

With this solution, the south pole of the permanent magnets stays longer in time (twice) over the feeder sensor.

·      The sensor “sees” the two or four permanent magnets as a unit of one permanent magnet; the signal is not pulsed!

 

The exchange of the magnet disc requires adjustments regarding the slide insert position and the magazine load position!

 

·      Adjust always the slide insert position first, and then check the magazine load action in relation to the found slide insert position.

 

The disc modification or exchange does not require software modification; so the upgrade may be executed regardless the software version.

 

 

 

 

 

 

 

 

 

 

 

 

Components and construction of the slide loader

 

  The slide loader is mounted on the magazine unit but is not a part of the magazine unit; it is a separate spare part instead.

 

 

·       Nevertheless, adjustments may be required if the slide loader is exchanged!

 

The slide loader movable part has only two positions, the inner and the outer position. The entire mechanics is constructed so, that only these two positions are defined. The state “Inner position reached” is signaled to a sensor via the “Sensor operating rod”.

 

 

 

 

 

 

 

 

      Early version of slide loader

 

 

The DC motor drives via a gear the spindle and so the moveable part of the slide loader. In the clutch housing the connection between gear and spindle is realized. This way, the inner and outer finger can be moved into the specimen holder of the X-Y-stage to insert a slide, or return, from the specimen holder to the slide position of the magazine, to remove a slide.

 

 

       DC controller

 

 

 

 

 

Because the mechanical construction has inertia, an inner and outer position fixing was realized. This solution eliminates the inertia and guarantees always the same limit position for the inner and outer position of the slide loader moveable part. The slide loader movable part reaches always the outer position fixing, but the inner position fixing is a backward movement limiter only (against inertia); it is not a requirement that the inner position fixing is reached always.

 

·        During the inner sensor stays active if the inner position is reached, the outer position sensor stays released when the outer final position is reached.

 

       Check the slide loader sensors

 

 

 

 

      Newer version of the slide loader

 

 To increase the stability of the slide insert procedure the construction of the slide loader was modified since the software version 1.16.

The inner and the outer sensors are situated in the clutch housing. The “Sensor operating rod” removes the sensor magnet (situated on the sensor spring 1) from the Hall element for the inner sensor.

This event is signaled to the software and the software will switch off the DC motor, the inner position is reached.

If the moveable part of the slide loader moves outward, from the inner position to the outer position, the magnet, situated on the sensor spring 2 will be moved over its Hall element for the outer sensor; this state is signaled to the software and the motor will be switched off. A spring (not shown) in the outer end position moves the slide loader moveable part into the “outer position fixing” by the help of the "Outer sensor operator"; releases so the sensor and the final outer position is reached.

 

·       The slide leader is no longer used (since the software version 1.16); its task is replaced by the "Slide leading spring" together with the "Slide leading edge" and the shape of the inner and outer finger.

·       Please take care on the "Sensor cover", it may be deformed easily!

 

 

 

 

 

 

Since the summer of the year 2014 the same construction of the specimen holder and slide insert removal mechanics is used as in the P250.

 

·      Only mechanical modifications existing on the moveable part (Slide clamp lifter and inner finger) the main construction and working principles are not changed.

 

·      For related components and adjustments, please refer also to relevant chapters in the P250.

 

       Magazine unit of P250; Slide loader; Adjustment procedures

 

 

 

 

 

 

 

 

 

 

 

 

 

      Limit positions

Because the mechanical construction has inertia, an inner and outer position limit was realized. This solution eliminates the inertia and guarantees always the same limit position for the inner and outer position of the slide loader moveable part. The slide loader movable part reaches always the outer position limit, but the inner position limit is a backward movement limiter only (against inertia); it is not a requirement that the inner position limit is reached always.

 

·        During the inner sensor stays active if the inner position is reached, the outer position sensor stays released when the outer final position is reached.

 

          Check the slide loader sensors

 

 

 

 

 

 

 

 

      Components

 

The inner and the outer sensors are situated in the clutch housing. The “Sensor operating rod” removes the sensor magnet (situated on the sensor spring 1) from the Hall element for the inner sensor.

This event is signaled to the software and the software will switch off the DC motor, the inner position is reached.

If the moveable part of the slide loader moves outward, from the inner position to the outer position, the magnet, situated on the sensor spring 2 will be moved over its Hall element for the outer sensor; this state is signaled to the software and the motor will be switched off. A spring (not shown) in the outer end position moves the slide loader moveable part into the “outer position fixing”; releases so the sensor and the final outer position is reached.

 

 

  • Please take care on the "Sensor cover", it may be deformed easily

 

 

 

 

 

 

 

 

 

 

 

 

 

 

If the moveable part of the slide loader moves outward, from the inner position to the outer position, the "Outer sensor operator" moves the magnet, situated on the sensor spring 2 will be moved over the Hall element for the outer sensor. This state is signaled to the software and the motor will be switched off. A spring (not shown) in the outer sensor operator moves the slide loader moveable part into the “outer limit position”; releases so the sensor and the outer position is reached. While the inner sensor stays active if the inner position is reached, the outer sensor stays released when the outer position is reached.

 

·       The sensor state on the right is shown in the final outer position

 

          Check the slide loader sensors

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                         Handling the slide loader

 

The slide loader should be hold at the motor and / or the clutch housing, but please does not touch the sensor cover, it may be deformed easily.

 

 

Move the slide loader moving part manually

 

For test purposes, the moving part of the slide loader can be moved manually also. If the slide loader cable HMJ-1 is disconnected, the movement is done easier.

The slide loader should be hold at the motor and / or the clutch housing, but does not touch the sensor cover, it can be deformed easily. The moveable part can be pulled on the mounting bolt of the inner finger or pushed on the surface near to the inner finger or outer finger mounting.

 

  • To avoid deforming, other parts should not be touched for this purpose.

 

 

 

 

 

 

 

 

                              Check the slide loader sensors

To check the inner sensor state, move the slide loader into the inner end position by clicking the right hand arrow button of the “Slide loader in or out” part of the service program. If the end position is reached, the sensor state should be attached and stays attached, as signaled by the sensor state “slide loader arm in the scope” (see below).

 

To check the outer sensor move the slide loader moveable part backward by clicking the left hand arrow button several times of the “Slide loader in or out” part of the service program.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

By pushing the slide loader moveable part backward manually and then releasing it, the sensor state “Slide loader arm out from the scope” should be attached and released either. Check this behavior several times.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                                      Faulty behavior

 

Push the moving part of the slide loader against the outer sensor operator manually. The sensor state is shown with, this means, the sensor is active.

Now release the slide loader moving part, the spring in the outer sensor operator should move the moving part to the outer limit position and the sensor state have to be X.

 

 

  • If the sensor stays attached after releasing it, faulty behavior occurred and the SlideScanner.exe program will not finishing the start up procedure!

 

  • If this behavior occurred, the moveable part of the slide loader moves to strong or the force of the spring in the outer sensor operator is too low.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Slide loader mounting

 

The slide loader is mounted onto the two side walls of the magazine channel as shown in the figure on the right. 

 

·       Both mountings include a horizontal and a vertical fixing for the slide loader position.

 

The exact horizontal position of the slide loader in relation to the specimen holder is defined by adjusting the entire magazine unit position with the “Magazine unit mounting” and the “Magazine unit adjustment” bolts respectively and was discussed above.

 

Because the “Slide loader inner finger” moves into the specimen holder, it’s height in relation to the slide clamp (the vertical position) must be adjusted also and will be discussed later.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

             Remove the slide loader

 

1.      Disconnect the Slide loader cable “HMF-1”.

2.      Move the slide loader moving part into the specimen holder manually, until the inner finger stays behind the slide clamp.

 

3.      Take the slide loader inner part from below (the part named as “2. PULL”) with your right hand and push the “Plastic Holder” (“1. PUSH”) with the thumb to the left a little bit, until the “Inner vertical fixing” is disconnected.

4.      Now pull the inner part (2= PULL) of the slide loader upward a little bit.

5.      Shift the entire slide loader frontward (3) a little bit to disconnect the “Outer vertical fixing”.

6.      Now the entire slide loader can be removed in upward direction (4).

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

             Mount the slide loader

 

7.      Move the specimen holder to Y-home 1,2 and X-home 1,2.

8.      Move the slide loader moveable part toward to the inner end position. The inner finger should be behind the “Slide clamp”, inside of the specimen holder.

9.      Fit the slide loader with the “Slide loader mounting 2” so, that the “Compression spring” (see above) is pressed and push the slide loader motor or the clutch housing downward, but do not press the sensor cover, it can be deformed easily. To mount this part easier, you can insert it a little bit slanted and by rotating it into the vertical position the spring can be strained easily, see also “Mount the slide loader 1 and 2”.

 

 

 

 

 

  • Insert the slide loader outer part slanted, and then push the outer part into the outer mounting. This way, the compression spring may be stressed easier.
  • Take care on the sensor cover, please do not deform it!

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

  

 

 

10. Close the gap between clutch housing and magazine feeder channel wall so that the vertical fixing is connected right.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

11. Push the slide loader to the left and downward until the horizontal and vertical position fixing of the “Inner slide loader mounting” is connected alright; see “Mount slide loader 3”. Some times it helps if the slide loader clutch housing is pushed to the magazine channel side wall at the same time.

 

  • The slide loader is connected well if the horizontal and vertical fixings on both sides of the slide loader are connected right and no significant gaps are visible.

 

 

  

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

               Slide handling parts

 

Early version of slide loader

 

The slide leader has two useful surfaces during slide loading. The “Slide leading surface” brings the slide in vertical position from the left side during slide loading; and the “Slide holding surface” presses the slide into the magazine rail with the force of the slide leader spring, to fixing the slide in vertical position. Meanwhile slide removal, the slide holding surface pushes the slide into the slide rail of the magazine.

 

Used only in the version:  LoaderType = SL_6Mag_25Slide_NoSensor_Vertical; until the software version 1.14.

 

          Configure the magazine unit” and “The magazine load procedure

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Specimen fingers until SW version 1.14

 

The shape (1) of the outer finger helps to positioning the slide into the middle of the outer finger (it should be the middle of the magazine rail also) if the outer slide edge is attached during slide insertion.

  • During slide removal this part of the outer finger has no function.

 

In case of slide insertion the inner finger does not touch the slide, but it helps with the edge (3) to open the slide clamp of the specimen holder. This way, the slide insert procedure is done easier.

During slide removal the surfaces (2) touches the upper and the lower part of the inner slide edge.

 

  • By adjusting the height of the inner slide finger, the slide clamp of the specimen holder will be opened, even if the widest and thickest allowed slide sizes are loaded.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Adjust the inner finger vertical position until SW version 1.14

 

 

By loosening the mounting bolt of the slide loaders inner finger, its position can be adjusted. The adjustment is correct, if the inner finger even passes the slide leader bolt without hitting it. By moving the slide loader moveable part manually, the inner finger should even pass the bolt for the slide leader up or down movement. If the distance is too much, the inner finger can hit the slide clamp of the specimen holder if the slide loader moves backward after slide insertion.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Specimen fingers

 

These specimen fingers are used on produced scanners since the SW version 1.16 (October 2012).

The shape (1) of the outer finger helps to positioning the slide into the middle of the outer finger (it should be the middle of the magazine rail also) if the outer slide edge is attached during slide insertion.

  • During slide removal this part of the outer finger has no function.

 

In case of slide insertion the inner finger does not touch the slide.

During slide removal the surfaces (2) touches the upper and the lower part of the inner slide edge.

 

  • By adjusting the height of the inner slide finger, the slide clamp of the specimen holder will be opened, even if the widest and thickest allowed slide sizes are loaded.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

      Height of the inner finger

The height of the inner finger is correct, if the inner finger crosses even the statically part of the slide loader, during moving to the outer end position.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

      Slide loader and slide clamp

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

  • The “Slide clamp lifter ball bearing” moves on the runway of the “slide clamp lifter” with a very little mechanical resistance and, as defined by the shape of the runway of the clamp lifter, it lifts up the slide clamp until the first part (the inner edge) of the slide is fully inserted.
  • Then it lowers down the slide clamp again. Remember, the slide may differ in length, width and thickness.

 

    Slide dimensions.

 

 

 

 

 

 

 

 

 

  

 

 

                   Further slide handling parts

 

The leading of the slide from the magazine to the specimen holder during the slide insert action requires special slide leading means.

 

The successful slide insert operation depends on:

  • The correct position of the magazine unit; so the slide leading edge of the slide position guide will be in plane with the right hand edge of the specimen holder; the inner finger of the slide loader even passes the specimen holder’s right hand edge.
  • The correct height of the slide loader’s inner finger; so the slide clamp may be opened correctly to insert the widest slides also.
  • The correct distance of the slide leading spring to the slide leading edge (about 1.4mm); so the slide will be moved to the slide leading edge and will be shifted on it.

 

 

1= Magazine supporter

2= Specimen holder fixing fork

3= Slide leading spring with holder

4= Slide position guide

5= Inner slide loader fixing

 

 

 

 

 

 

 

 

Slide leading spring 

The slide leading spring leads the slide to the slide leading edge of the slide position guide and by shifting the slide along the leading edge, the horizontal position of the slide is defined.

 

Slide position guide 

 

The slide position guide defines with the slide leading edge the horizontal position of the slide during slide insertion.

The slide leading slot bottom is used to define the vertical position of the slide during slide insertion.

 

  • During slide removal the parts have no function.

 

Specimen holder fixing fork 

 

During slide insertion and slide removal, the Specimen holder fixing fork fixes the horizontal position of the specimen holder. This is mainly important for a successful slide insert operation.

 

          P250 Specimen holder fixing fork

 

 

 

 

 

 

 

 

 

 

Slide leading spring 

 

In the non-working position, the angulated end of the slide leading spring must not hit the inner side wall; only this way a deforming of the spring can be avoided.

 

 

  • The non-working position is reached if the slide loader stays in the outer position.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

In the working position, the distance of the spring to the slide leading edge is important.

If we assume, that the maximal slide thickness is 1.20mm and the thickness of the cover slip is 0.17mm, the resulting value together with tissue will be 1.37mm, so about 1.40mm distance to the slide leading edge is required.

 

·                                           During slide insertion, the slide leading spring must not perform force against the cover slip.

 

The position of the slide leading spring is correct, if the distance to the slide leading edge is:

1.37mm ≤ distance to the slide leading edge ≤ 1.45mm; measured to the closest part of the spring.

 

 

 

 

Gap to the slide

For this measurement, please use a slide with a thickness of 1.20mm without cover slip.

 

0.2mm ≤ distance to the slide (without cover slip) ≤ 0.30mm; measured to the closest part of the spring.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Slide position guide

The slide leading edge and slot is situated in the inner side wall of the magazine channel. The slide “Leading Slot” bottom should be 0.1mm higher than the lower edge of the specimen holder during slide insertion. The slide “Leading edge” should be in the same plane with the specimen holder right hand edge; see also ‘Adjust the slide insert position in X-direction.

The shape of the “Leading slot”, the construction of the slide holding mechanics of the specimen holder (Slide clamp and lower edge) and the width of the slide position in the magazine are together the reason for the maximal allowed slide thickness of 1.05mm.

 

The slide clamp of the specimen holder, together with its lower edge holds the slide during scan operation or moving the specimen holder inside the scannable area. If the slide insert or removal position is reached, the slide clamp is loosened via the slide tightener; only the small force of the “Soft Spring” situated behind the slide clamp will hold the slide clamp and the slide can be moved easily.

 

If the slide insert or removal position will be reached, the Slide tightener touches the magazine feeder channel from below meanwhile the specimen holder moves in upward direction and forces so the “Strong spring”, situated behind the slide clamp to release the slide clamp (details about the specimen holder mechanics are discussed in the file “X-Y-stage”).

 

  • During slide insert operations, the right hand edge of the specimen holder has to be in the same plane as the slide leading edge.

 

 

 

 

 

 

 

Slide insert and removal

 

The following steps describe the slide insert and removal procedure as it is done by software; until the software version 1.14.

 

Slides insert procedure

 

1.      The focus pin goes to the position +800 steps to bring the specimen holder into the nominal slide insert position horizontally (Z-direction). In this position the slide “Leading edge” of the slide position guide should be in the same vertical plane with the “Right hand edge” of the specimen holder.

 

2.      The specimen holder goes to the slide insert position in X (InsertSlidePositionX) and Y (InsertSlidePositionY1) direction. The value for the slide insert position in Y direction is always +400 steps.

 

3.      Meanwhile the specimen holder moves upward (-X direction), the slide tightener will be moved to the lower edge of the magazine feeder channel and releases so the slide clamp; it will be hold only by the force of the soft spring; the slide can be moved easy into the specimen holder.

 

4.      If the slide insert position is reached, the slide loader motor is started with a time of 600ms. The outer finger touches the slide edge and moves the slide in direction to the specimen holder; meanwhile the shape of the outer finger  helps to find the middle of the rail.

 

5.      A few 100 ms later, the slide leader moves downward to the upper edge of the slide and brings so the slide into vertical position with the help of the “Slide leading surface”; then the “Slide holding surface” holds the slide on the upper edge with the force of the “Slide leader spring”.

 

6.      The “Slide leading slot” leads the slide lower part to the specimen holder “Lower edge” and the “Leading edge” defines the position of the slide in relation to the “Right hand edge” of the specimen holder; both should be in the same plane.

 

7.      If the inner finger arrives to the slide clamp of the specimen holder, the edge  and the shape of the slide clamp is opening the slide clamp and the first part of the slide will be inserted; meanwhile the slide leader releases the upper edge of the slide.

 

8.      If the time of 600ms is over, the specimen holder moves to the final Y-position (InsertSlide PositionY2, 3). Both parameters have always the same value.

 

9.      The slide loader motor is started again until the inner sensor of the slide loader is attached. The software will switch off the motor and the inner sensor stays attached; the slide is inserted.

 

10.  Now the specimen holder moves with the slide in +X direction (downward) with the value of the parameter DeafultPositionX nearly to the limit Xmax.

 

11.  The slide loader motor is started again, but now in opposite direction until the slide loader outer sensor is attached and the slide loader motor will be switched off by software. A spring (not shown) moves now the slide loader moveable part to the outer position fixing, meanwhile the outer sensor will be released; the software checks the released state of the sensor after 300 ms (SlideLoaderDelayTimeBeforeSensor) and the slide insert procedure is finished or, if the sensor is not released or the sensor was not attached and the time was run out, the message “Slide loader arm got stuck during process!” will be issued.

 

 

 

 

Slide removal procedure

 

1.      The focus pin goes to the position +800 steps to bring the specimen holder into the nominal slide removal position horizontally (Z-direction).

 

2.      The specimen holder goes to the positions X-Home1,2 and Y-Home1,2.

 

3.      To remove a slide, the specimen holder moves first with the slide in +X direction (downward) with the value of the parameter DeafultPositionX nearly to the limit Xmax. The correct Y-remove position is defined with the value of the parameter RemoveSlidePositionY.

 

4.      The software will switch on the Slide Loader motor until the inner sensor will be attached.

 

5.      Now the specimen holder goes in –X direction (upward) and moves so the slide between the inner and outer finger of the slide loader. The end position is defined by the value of the parameter RemoveSlidePositionX1.

 

6.      Meanwhile the specimen holder moves upward (-X direction), the slide tightener will be moved to the lower edge of the magazine unit and releases so the slide clamp; the slide will be held by the Slide clamp with the force of the soft spring only.

 

7.      The Slide Loader motor is switched on in backward direction for 300ms and then stops the movement; the surfaces  and  of the Inner Finger touches the slide inner edge and moves so the slide backward; the outer part of the slide stays over the magazine rail; the inner finger stays inside the specimen holder before the slide clamp and the slide leader does not touch the slides upper edge.

 

8.      Now the specimen holder goes downward to the position, defined by the value of the parameter RemoveSlidePositionX2 and places so the slide outer part over the bottom of the magazine rail.

 

9.      The slide loader motor is switched on again in backward direction; the slide will be pushed into the rail of the slide position of the magazine by the slide leader nearly until the outer sensor is attached; the slide loader motor will be switched off by the software. At the end of this procedure a spring (not shown) moves now the slide loader moveable part to the outer position fixing, meanwhile the outer sensor will be released and the software checks the released state of the sensor after 300ms (SlideLoaderDelayTimeBeforeSensor). The slide removal procedure is finished.

 

10.  With the magazine feeder, the next or any slide position in the magazine can be found to select the slide to be inserted next.

 

If the “LoaderType” is:

 

LoaderType = SL_6Mag_25Slide_NoSensor_Vertical_2;     the magnet disc stops with the rising edge of the sensor

 

          Slide insert and removal” procedure of the P250!

 

 

 

 

 

 

 

 

 

Magazine load procedure

 

The magazine load procedure is done in 2 ways, depending on the value of the parameter “LoaderType” in the file MicroscopeConfiguration.ini section [Microscope].

 

The following procedure is true, if the value is

 

LoaderType = SL_6Mag_25Slide_NoSensor_Vertical

 

Or the parameter does not exist in the file MicroscopeConfiguration.ini section [Microscope].

 

           Configure the magazine unit

 

1.      If the program SlideScanner.exe starts up, the following actions are done: 

a.      The slide loader is moved backward, to the outer fixing position.

b.      The feeder gear had done at least one turn backward; the sensor disc stays in the magazine load position; see also “The magnet disc”.

 

2.      The magazine load procedure is started via SlideScanner.exe (or the service program) with the button “Load Magazine”.

 

3.      The magazine load motor and the input stack wheels are driven forward and the magazine moves downward to the magazine feeder rails. At the same time, the magazine (if any) is moved from the feeder channel to the output stack.

 

4.      If the magazine input wheels have done a half turn, the magazine load sensor will be attached and the software will switch off the magazine load motor.

 

5.      At the same time, as the magazine arrives onto the magazine feeder rails the magazine input sensor will be attached; so the software knows that a magazine is present on the magazine feeder rails (see also the description “Magazine input sensor”).

 

6.      The software starts the magazine feeder gear one turn backward (to the left) and checks the state of the left magazine sensor (see also “The Magazine input sensor”). The sensor should not be pressed.

 

7.      If the sensor is not pressed, one turn forward (the magazine moves to the right) of the feeder gear follows and the sensor should be pressed again. This way, the software checks the function of the sensor and the presence of the magazine.

 

8.      Now the magazine is moved to the actual slide position, shown by the slide position selector of SlideScannerScan.exe.

 

 Magazine unload procedure

 

1.      If the last slide to be scanned had been removed into the magazine, the magazine feeder gear moves the magazine to the right (forward), until the magazine output sensor was released.

 

2.      The software counts the pulses of the magnet disc of the magazine feeder and checks always the state of the right hand sensor (Magazine output sensor).

 

3.      If the state of the magazine output sensor is released, a turn backward (the magazine moves to the left) follows and the magazine output sensor is checked again.

 

4.      If the sensor is pressed, the magazine load motor is started, the output stack wheels rotates for a half turn and moving so the magazine into the output stack; in the same time the next magazine (if any exists) is moved from the input stack onto the feeder rail.

 

5.      The software checks the magazine input sensor (left magazine sensor). If it is not attached (see also the description “Magazine input sensor”), the magazine load and unload procedure is finished; otherwise go to step 5 of the “magazine load procedure”.

 

 

 

 

The following procedure is true, if the value is

 

                                                                  LoaderType = SL_6Mag_25Slide_NoSensor_Vertical_2

 

 

          Configure the magazine unit

 

 

  1. If the program SlideScanner.exe starts up, the following actions are done: 
    1. The slide loader is moved backward, to the outer fixing position.
    2. The feeder gear had done at least one turn backward; the sensor disc stays in the magazine load position; see also “The magnet disc”.

 

  1. The magazine load procedure is started via SlideScanner.exe (or the service program) with the button “Load Magazine”.

 

  1. The magazine load motor and the input stack wheels are driven forward and the magazine moves downward to the magazine feeder rails. At the same time, the magazine (if any) is moved from the feeder channel to the output stack.

 

  1. If the magazine input wheels have done a half turn, the magazine load sensor will be attached and the software will switch off the magazine load motor.

 

 

Until now, all is the same as before; the modification follows now.

                                                         

  1. The magazine input sensor may be pressed or not, the software changes the position of the magnet disc from “Stop backward” to “Stop forward” first, before the state of the magazine input sensor is checked!

If the magazine input sensor stays attached, the magazine will be feed to the first (or the actual) slide insert position.

If the magazine input sensor stays released, the state “Magazine not present” will be detected and all further operations are aborted.

 

 

Magazine unload procedure

 

  1. If the last slide to be scanned had been removed into the magazine, the magazine feeder gear moves the magazine to the right (forward), until the magazine output sensor was released.

 

  1. The software counts the pulses of the magnet disc of the magazine feeder and checks always the state of the right hand sensor (Magazine output sensor).

 

  1. If the state of the magazine output sensor is released, a turn backward (the magazine moves to the left) follows and the magazine output sensor is checked again.

 

  1. If the sensor is pressed, the magazine load motor is started, the output stack wheels rotates for a half turn and moving so the magazine into the output stack; in the same time the next magazine (if any exists) is moved from the input stack onto the feeder rails.

 

  1. The software checks the magazine input sensor (left magazine sensor). If it is not attached (see also the description “Magazine input sensor”), the magazine load or unload procedure is finished; otherwise go to step “E” of the “magazine load procedure”.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Define and adjust the slide insert and removal positions

 

The task is, to define the parameter values mentioned below, and to do the required adjustments so, that the slides insert and removal procedure is done evenly and smoothly, without slide jamming or even slide cracking. Furthermore, the slide insert or removal action, done by software, should not cause collision of the slide loader inner finger with the slide clamp of the specimen holder; even if there was no slide in the magazine and a slide removal action is executed. The following adjustments assume that the focus unit is adjusted well and the hardware limits for the X-Y-stage are already defined.

 

The slide insert position adjustment is done in the following steps and with the following parts:

 

1.      Adjust the position of the magazine unit; so the position of the slide loader and the “Slide leading edge” in the side wall of the magazine channel in relation to the right hand edge of the specimen holder will be adjusted.

 

2.      Adjust the position of the magnet disc; so the position of the slide loader outer finger in relation to the magazine rail will be defined. The outer finger and the slide should be in the middle of the magazine rail.

 

See also “Configure the magazine unit” and “The magazine load procedure

 

3.      Adjust the position of the inner finger of the slide loader and define the value of the parameter “DeafultPositionX” (See also above “To adjust the position of the inner finger” in the description of the slide loader). This adjustment guarantees that the inner finger can cross the slide clamp of the specimen holder in the step 11 of the slide insert procedure and in the step 4 of the slide removal procedure.

 

4.      Define the value of the parameterDeafultPositionX

If the X-stage is in the position “DeafultPositionX”, the slide loader inner finger should be able to cross the slide clamp of the specimen holder without touching it. For this adjustment insert a slide with the maximal width of 26.00mm. Check this behavior manually first, then electromechanically by moving the slide loader moveable part. If hitting occurs, check the position of the inner finger, see also “To adjust the position of the inner finger” in the description of the slide loader and check the correctness of the parameter value ObjectGuideXMax.

 

5.      Adjust the slide insert position in X-direction for the specimen holder; so the position of the lower edge of the specimen holder in relation to the slot of the slide leading guide will be defined. The conclusion of this adjustment is the value of the parameter InsertSlidePositionX”.

 

 

6.       Adjust the final insert position in Y-direction of the specimen holder; define the value of the parameters “InsertSlidePositionY2,3”. The goal of this adjustment is, that:

a.      The slide loader outer finger never touches the slide clamp of the specimen holder during slide insertion and

b.      The slide never touches the side wall of the magazine channel, even if the specimen holder is in the Y-position -800 steps and is moved in X-direction (the Y-position of -800 steps is used to capture the preview).

 

Steps for slide removal:

 

1.      Define the value of the parameter RemoveSlidePositionY.

 

2.      Define the value of the parameter RemoveSlidePositionX1

 

3.      Define the value of the parameter RemoveSlidePositionX2

 

            Adjust the magazine unit

 

 

 

 

Adjust the position of the magazine unit

 

Task: The slide, if shifted backward from the specimen holder even touches the slide leading edge.

 

1.      See also the description “The magazine unit position adjustment” in the chapter “Components and mechanical construction of the magazine unit”.

 

2.      Set the focus position to +800 steps, the nominal slide insert position in Z-direction.

 

3.      Insert a slide manually and the specimen holder should be in the position Y home 1,2 and X home 1,2.

 

4.      Shift the slide manually and carefully from the specimen holder backward, in direction to the magazine until the slide leading edge will be touched. The position of the specimen holder (Z-direction) should not be changed during this action!

 

5.      Check and adjust the magazine unit position so, that the slide leading edge is even touched by the slide.

 

6.      Shift the slide manually return into the specimen holder so, that the specimen holder can move downward without slide cracking. With the service program go downward 26000 steps in +X direction and return to the actual X position. In this case, the slide tightener is disconnected from the magazine unit and the done adjustment becomes effect. This step 6 should be done after each magazine unit movement, even if only small adjustments are done!

 

7.      Repeat steps 4 to 6 until the requirements are fulfilled.

 

8.      Check this adjustment again if the procedure “Adjust the position of the magnet disc” had been done.

 

 

 

 

 

 

 

 



 

 Adjust the position of the magnet disc

 

Task:  The slide, if shifted backward from the specimen holder fits the middle of the magazine rail.

 

1.      See also the description “The magnet disc in the chapter “The magazine feeder part”.

 

2.      Check the stop position of the magnet (disc) in relation to the Hall element and memorize it.

 

3.      Set the focus position to +800 steps, the nominal slide insert position in Z-direction.

 

4.      Insert a slide manually into the specimen holder so, that the outer part of the slide stays over the magazine rail.

 

5.      Adjust the magnet disc position on the feeder spindle by rotating the feeder spindle manually so, that the slide is in the middle of the magazine rail, and the magnet disc is in the previously checked, memorized stop position.

 

6.      Check this adjustment in the positions 2, 13 and 24 of the magazine and correct the found position, if necessary. 

 

7.      Check this adjustment again if the procedure “Adjust the position of the magazine unit” had been done.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Check and adjust the magazine position

 

 Task

  • Adjust the slide position of the magazine so, that the outer specimen finger is in the middle of the slide slot of the magazine.
  • The adjustment is done by positioning the The magnet disc” on the feeder spindle.

 

The adjustment assumes that the position of the magazine unit is correct.

1.      Insert a magazine manually or by the use of the service program, prepared with two slides, nearly in the middle of the magazine as shown on the right (“Magazine”).

2.      Set the feeder rising or falling edge as required

3.      With the part “magazine feeder” of the service program move the magazine in the feeder channel until the slide loader stays over the “empty position”, between the two slides, and the stop position of the magnet disc is “Stop forward”, the slide insert position.

4.      Move the slide loader moving part manually a little bit into the scanner until the outer finger can be seen easily.

5.      Push the two slides toward the outer finger.

6.      If the gaps on both sides of the outer finger are not equal, memorize the “Stop forward” position then rotate the magnet disc until the gaps are equal. Memorize this position also.

7.      Loosen the magnet disc fixing and rotate the magnet disc by the difference of the two memorized positions and tighten the fixing bolt.

8.      With the service program move the slide loader moving part into its outer end position, rotate the feeder gear backward two turns, then rotate the feeder gear forward until the empty position of the magazine is found again, the stop position of the magnet disc is “Stop forward”.

9.      Repeat from step 4 until the gaps are equal on both sides.

If the adjustment is finished, check the correctness of the found position by executing the magazine load test. Check all the magazines without any slide in any magazine; see also: “Check the magazine load procedure”.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Adjust the slide insert position in X-direction

 

 Requirements

Two slides with a thickness of 1.05mm, equal in shape, without cut corners.

 

Task:  Find the height of the slide in the specimen holder in relation to the slide, pushed from the magazine; the height should be equal at the shown position. If this position of the specimen holder in X-direction is found, add to the actual X-position 100 steps and set the value for the parameter InsertSlidePositionX.

 

 

1.      Set the positions Y-Home1,2 and X-Home1,2 of the X-Y-Stage.

 

2.      Set the focus position to +800 steps, the nominal slide insert position in Z-direction.

 

3.      Set the X-direction to the value of 1600 steps.

 

4.      Set the Y-direction to the value of the parameter InsertSlidePositionY1 (=400 steps).

 

5.      Remove the slide loader and insert a slide without cut corners manually into the specimen holder.

 

6.      In the magazine position behind the specimen holder insert a second slide of the same shape and the same size.

 

7.      Push the slide in the magazine position forward to the outer edge of the inserted slide.

 

8.      Modify the specimen holder X-position (service program, X-direction) so, that both slides are equal in height. You can check this by using a screw driver and moving it on the upper edge of both slides, over the fitting edge. If the height of both slides is not equal, a step can be recognized. An accuracy of 100 steps is enough.

 

9.      If the height of both slides is equal add to the found value 100 steps and the value of the parameter InsertSlidePositionX is found.

 

10. Update the value of the parameter InsertSlidePositionXin the fileMicroscopeConfiguration.iniand save the file.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Set the value of “InsertSlidePositionY1’

 

The value is set to be -500steps. If this limit is less then the hardware limit “ObjectGuideYMin”, please use a value of

 

InsertSlidePositionY1 = ObjectGuideYMin + 100steps

 

In earlier released SCAN versions, this value were set to +400steps.

 

·       The value is not critical in question of slide operations.

 

 

 

 

 

 

 

 

 

 

Adjust the final insert position in Y-direction

 

 Task:  Set the value for the parameters “InsertSlidePositionY2,3” so, that the specimen holder is never touched. Remember, that both parameters must have always the same value!

1.      Set the positions Y-Home1,2 and X-Home1,2 of the X-Y-Stage.

 

2.      Set the X-direction to the value of the parameter InsertSlidePositionX.

 

3.      Set the Y-direction to the value of the parameter InsertSlidePositionY1 (=400 steps).

 

 

4.      Push the slide loader moveable part manually, until the slide clamp of the specimen holder is reached by the outer finger.

 

5.      Adjust the specimen holder Y-position (service program, Y-direction) so, that the outer finger of the slide loader has a gap of about 0.2 mm (means 200steps) to the slide clamp of the specimen holder and the slide loader moveable part is fully pushed into the inside of the specimen holder. An accuracy of 100 steps is enough.

 

6.      The right value for the parameters InsertSlidePositionY2,3is found if the slide loader is fully pushed into the specimen holder (check this behavior manually) and a minimal gap is given between slide loader outer finger and slide clamp of the specimen holder. If the gap is too much, the slide will touch the side wall of the magazine channel if the specimen holder is in the Y-position -800 steps (used for the preview capture) and the slide is moved in X-direction.

 

7.      Update the value of the parameters InsertSlidePositionY2,3in the fileMicroscopeConfiguration.iniand save the file.

 

 

 

 

 

 

 

 

 

Define the value of the parameter “DeafultPositionX

 

DeafultPositionX = ObjectGuideXMax  -100steps

 

Update the value of the parameter DeafultPositionXin the fileMicroscopeConfiguration.iniand save the file.

 

 

 

 

 

 

 

 

Define the value of the parameter RemoveSlidePositionY

 

Add six hundred steps to the value of the parameter InsertSlidePositionY2,3”.

 

RemoveSlidePositionY = InsertSlidePositionY2,3 + 600steps

 

Update the value of the parameter RemoveSlidePositionYin the fileMicroscopeConfiguration.iniand save the file.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Define the value of the parameter RemoveSlidePositionX1

RemoveSlidePositionX1 = InsertSlidePositionX -1000steps or, if impossible, the limit X-min.

 

Update the value of the parameter RemoveSlidePositionX1in the fileMicroscopeConfiguration.iniand save the file.

 

 

 

 

 

Define the value of the parameter RemoveSlidePositionX2

 

1.      Define the value for the parameter RemoveSlidePositionX2” (the height of the specimen holder in X-direction) so, that the Edge (3) of the inner finger crosses the slide clamp easily, if no slide is inserted.

 

2.      Update the value of the parameter RemoveSlidePositionX2in the fileMicroscopeConfiguration.iniand save the file.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Adjust the magazine load mechanics

Since the software version 1.15 the magazine feeder disc movement is stopped with the rising edge of the magazine feeder sensor, depending on the magazine unit type, see “Configure the magazine unit” and see also above “Magnet disc”.

 

In this case of “LoaderType = SL_6Mag_25Slide_NoSensor_Vertical_2;  the magnet disc stops with the rising edge of the sensor; therefore, please refer to the chapter “Adjust the magazine load mechanics” of the file “P250_Magazine_unit_slide_handling”.

 

The following part is true, if the LoaderType  is LoaderType = SL_6Mag_25Slide_NoSensor_Vertical;         if the magnet disc movement is stopped with the falling edge of the magazine feeder sensor.

 

         Configure the magazine unit” and “Magnet disc”.

 

In this case of “LoaderType = SL_6Mag_25Slide_NoSensor_Vertical_2;  the magnet disc stops with the rising edge of the sensor; therefore, please refer to the chapter “Adjust the magazine load mechanics” of the file “P250_Magazine_unit_slide_handling”.

 

The following part is true, if the LoaderType  is LoaderType = SL_6Mag_25Slide_NoSensor_Vertical;         if the magnet disc movement is stopped with the falling edge of the magazine feeder sensor; see “Configure the magazine unit” and also above “Magnet disc”.

 

 

1.      Start the service program SlideScannerService.exe and switch active the section “Low level service” and “DC controller”.

 

2.      Select the “rising” edge of the “Magazine feeder sensor”

 

3.      With the “Magazine Feeder” backward button the feeder gear brings the magnet disc into the start position for magazine load.

 

4.      With the “Magazine loader” forward button bring the magazine input and output stack wheels into the start position.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

5.      Check the “Magazine loader” sensor state and the “Magazine Feeder” sensor state as shown on the right.

 

        Configure the magazine unit” and “Magazine load procedure

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

6.      Check the stop position of the input and output stack wheels.

The stop position of the stack wheels should be perpendicular, and in plane with the bottom of the magazine channel.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

7.      Adjust the magazine loader sensor position so, that this requirement (step 5) is reached, it is not a critical adjustment; repeat steps 3 to 6 until the requirements are fulfilled.

 

8.      Insert a magazine into the input stack and with the “Magazine loader” forward button start a magazine load. The magazine should move smoothly and evenly downward, without stumble.

 

9.      If stumble occurs, the magazine feeder gear should be adjusted via the magnet disc so, that the cogs of the feeder wheel and the magazine rack engages and do not disturb each other.

 

10.  If the magazine arrives onto the feeder rails, the magazine input sensor should be pressed (see also “Magazine input sensor”).

 

11.  Repeat the steps 7 to 9 several times and with different magazines. If the requirements are fulfilled, the adjustment is finished.

 

12.  Finally test the magazine load and unload procedure at least with 6 different magazines, without slides. Observe the load procedure, the empty magazines should move downward smoothly and evenly and the magazine sensor should be pressed, if the magazine arrives onto the feeder rails.

 

 

 

 

 

 

 

 

 

 

Adjust the magazine load mechanics

 

Since the software version 1.15 the magazine feeder disc movement is stopped with the rising edge of the magazine feeder sensor, depending on the magazine unit type, see “Configure the magazine unit” and see also above “Magnet disc”.

 

In this case of “LoaderType = SL_6Mag_25Slide_NoSensor_Vertical_2;  the magnet disc stops with the rising edge of the sensor; therefore, please refer to the chapter “Adjust the magazine load mechanics” of the file “P250_Magazine_unit_slide_handling”.

 

The following part is true, if the LoaderType  is LoaderType = SL_6Mag_25Slide_NoSensor_Vertical;         if the magnet disc movement is stopped with the falling edge of the magazine feeder sensor; see “Configure the magazine unit” and also above “Magnet disc”.

 

 

13.  Start the service program SlideScannerService.exe and switch active the section “Low level service” and “DC controller”.

 

14.  Select the “falling” edge of the “Magazine feeder sensor”

 

15.  With the “Magazine Feeder” backward button the feeder gear brings the magnet disc into the start position (not shown).

 

16.  With the “Magazine loader” forward button bring the magazine input and output stack wheels into the start position.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

17.  Check the “Magazine loader” sensor state and the “Magazine Feeder” sensor state, both should be attached.

 

          Configure the magazine unit” and “Magazine load procedure

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

18.  Check the stop position of the input and output stack wheels.

The stop position of the stack wheels should be perpendicular, and in plane with the bottom of the magazine channel.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

19.  Adjust the magazine loader sensor position so, that this requirement (step 5) is reached, it is not a critical adjustment; repeat steps 3 to 6 until the requirements are fulfilled.

 

20.  Insert a magazine into the input stack and with the “Magazine loader” forward button start a magazine load. The magazine should move smoothly and evenly downward, without stumble.

 

21.  If stumble occurs, the magazine feeder gear should be adjusted via the magnet disc so, that the cogs of the feeder wheel and the magazine rack engages and do not disturb each other.

 

22.  If the magazine arrives onto the feeder rails, the magazine input sensor should be pressed (see also “Magazine input sensor”).

 

23.  Repeat the steps 7 to 9 several times and with different magazines. If the requirements are fulfilled, the adjustment is finished.

 

24.  Finally test the magazine load and unload procedure at least with 6 different magazines, without slides. Observe the load procedure, the empty magazines should move downward smoothly and evenly and the magazine sensor should be pressed, if the magazine arrives onto the feeder rails.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Finishing the adjustment

 

 

                   1. Checks with the service program

Check the magazine load procedure by using empty, half filled and fully filled magazines (shown on the right).

 

The magazine load procedure should be executed without stumbling of the magazine; the magazines moving downward smoothly and the feeder wheel engages the rack of the magazine easily.

·       During this test, only 1 magazine should be in the input stack!

·       Check all the 6 empty magazines with this test.

·       Check with magazines, filled as shown on the right.

·       If stumble of the magazine during any load procedure occurs, please adjust the “Magazine position for the slide insert - removal operation” more precise.

·       Check also the magazine(s) and its rack.

 

During unload of the magazine, the magazine should move into the output stack smoothly and evenly. Arrived in the output stack, the magazine supporters should work correctly!

 

 

 

 

 

 

 

                         2. Check the magazine load operation with the test program

 

·       Use the magazines, prepared in the previous check also with the “Magazine load test”, the requirements are the same as above.

 

We assume the test “Check the magazine load - unload operation” is finished correctly.

 

Slide insert and removal test

Finally test the slide insert and removal actions with all the delivered magazines and different slide sizes and slide shapes. This test should include the thinnest, thickest, smallest and largest allowed (metal) slides. Test the slide insert or removal action also in magazine slide positions without slide.

 

Prepare the magazines for the test

Fill all available magazines with slides and execute the slide insert and removal test. In each magazine, there should be several places without slides in different slide positions.

 

 

During the slide loading test is in progress, check the following actions visually:

 

·       Please use always a protective eye wear if the slide loading test is in progress and you are looking closely at the loading procedure! It may happen that a slide can fly in peaces during the process, even if the adjustment is incorrect!

 

 

1.      During the magazine load action the magazines moving downward smoothly and evenly.

2.      The magazine input sensor is attached the magazine is moved to the first slide position (see also the chapter “Magazine input sensor”).

During slide insertion

3.      The slide is inserted smoothly and evenly, without bangs and stronger (more force requiring) sound, even if the thickest slides with the maximal width of 26.00mm are inserted.

 

4.      The smallest slides should be hold correctly.

 

5.      The outer finger does not touch the slide clamp of the specimen holder if the slide is fully inserted.

 

6.      If the slide loader moves backward the upper part of the slide clamp is not touched, even if slides with the maximal width of 26.00mm are inserted.

 

During slide removal

7.      The inner finger crosses the slide clamp if there was an empty position in the magazine.

 

     Mistakes and abnormal or faulty behavior during slide insertion

 

 

·       If all the tests and checks are fulfilled correctly, the adjustment of the magazine unit is finished.

 

 

 

 

 

Magazine load, unload and slide insert, removal test

 

 

Start the program “SlideScanner.exe” with the service password; the menu “Service” appears.

 

Select the option “Service” and “Slide loading test”; the combined “magazine load or unload” (1, 2) and the slide insert or removal (3, 4) test will be selectable.

 

·       Insert one or more magazine(s) into the input stack.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Magazine load - unload and slide insert – removal test

 

By pressing the button “Insert magazine” (1) the magazine is moved from the magazine input stack into the magazine feeder channel and if the magazine is detected by the left magazine sensor, the magazine is moved to the first slide position of the magazine.

 

By pressing the button Remove magazine” (2) the magazine is moved in direction to the output stack until the right magazine sensor is even passed, the sensor is in the released state.

The magazine feeder motor rotated the magnet disc one turn backward and checks the right magazine sensor again. If the right magazine sensor is attached, the magazine unload position is found and the magazine is moved into the output stack.

By repeating the procedure (press the buttons “Insert magazine” and “Remove magazine”) the magazine load test is performed.

 

 

 

By pressing the button Slide insert test” (3)

1.      If no magazine is present in the feeder channel:

The magazine load procedure is executed as described above (1).

The slides will be inserted into the specimen holder and a few seconds later it will be removed into the magazine.

If all (selected) slides of the magazine passed the slide insert and removal procedure, the magazine is feed to the output stack; see (2) above and the next magazine with slides will be loaded. The procedure will be repeated until magazines are present in the input stack or if the button “Stop test” (4) is pressed.

2.      If a magazine is already present in the magazine feeder channel:

The magazine is moved until the first slide position of the magazine is reached and then the slide insert - removal procedure is executed.

 

 

If the button Stop test” (4) is pressed, the test stops only, if the actual slide insert and removal action is finished.

In emergency conditions, the switch “Power off” of the power supply should be switched off.

 

 

 

Positions

In the table “Positions” slide positions of the magazine can be disabled from the slide insert and removal procedure by easily deleting the appropriate line from the table. If the test is loaded again from the start menu, all slide positions are available again.

 

 

 

 

                         Mistakes and abnormal or faulty behavior during slide insertion

 

The following or other faulty behavior can also occur if dust, paraffin residues or bits of broken glass are in the magazine slide rail or on other parts, needed for the slide insert and slide removal procedure. 

1.      The slide cannot be inserted, the slide hits the specimen holder and the action is aborted.

§           Check the plane of the slide leading edge with the specimen holder right hand edge (Magazine unit position; position of the specimen holder fixing fork).

§           Check the slide leading slot bottom in relation to the lower edge of the specimen holder (“InsertSlidePositionX”).

§           Check the position of the slide loader outer finger in relation to the magazine rail (Adjust the position of the magnet disc).

§           Check the slide dimensions of the used slide.

 

2.      Some slides are inserted well, but some times a big bang can be listened or even cracking the slide on the top:

§           Check the slide leading slot bottom in relation to the lower edge of the specimen holder; see also: “InsertSlidePositionX”.

§           Check the position of the slide loader outer finger in relation to the middle of the magazine rail.

§           Check the height position of the inner finger.

§           Check the slide dimensions of the used slide.

 

3.      Some slides are inserted well, but some times a big bang can be listened or even cracking the slide on the bottom:

§           Check the slide leading slot bottom in relation to the lower edge of the specimen holder.

§           Check the position of the slide loader outer finger in relation to the middle of the magazine rail.

§           Check the slide dimensions of the used slide.

 

4.      The slide insert procedure is done, but often or some times, the slide is slant inserted and falling down onto the truss.

§         Check the plane of the slide leading edge with the specimen holder right hand edge; see also “Adjust the magazine unit position”.

 

5.      During slide insertion a stronger (more force requiring) sound can be listened, even in the second phase of slide insertion or the slide is removed before the slide insert procedure is finished (the slide loader time out was running out).

§         Check or adjust the slide insert position in X-direction; define the value for the parameter “InsertSlidePositionX” again; the value of this parameter is often too much.

§         Check the slide dimensions of the used slide.

 

6.      If the slide will be inserted, a bang can be listened always or on some slides, the Inner finger cannot cross the slide clamp or is in collision with other parts of the specimen holder.

§         Check the plane of the slide leading edge with the specimen holder right hand edge.

§         Check the slide leading slot bottom in relation to the lower edge of the specimen holder (“InsertSlidePositionX”).

§         Check the height of the inner finger.

§         Check the slide dimensions of the used slide.

 

7.      The outer finger touches the specimen holder during the second part of slide insertion is done.

§         Check or adjust the slide insert position in Y-direction; define the value for the parameter “InsertSlidePositionY2,3” again.

 

8.      The slide is touching the sidewall of the magazine channel if the specimen holder is in the Y-position -800 steps.

§         Check or adjust the slide insert position in Y-direction; define the value for the parameter “InsertSlidePositionY2,3” again.

 

9.      If the slide loader is returned to the outer position (after slide insertion), the lower edge of the slide loader inner finger touches the upper part of the specimen holders slide clamp.

§         Define the value of the parameter “DeafultPositionX” again and check the height position of the inner finger; check also the correctness of the limit “Xmax”.

§         Check the slide dimensions of the used slide.

 

Mistakes and abnormal or faulty behavior during slide removal

 

10. The inner finger cannot cross the slide clamp if there was an empty position in the magazine.

§         Define the value of the parameter RemoveSlidePositionX2 again.

 

 

 

 

List of Parameters and explanation

 

In the following list the parameters for the magazine unit, the slide loading and slide removal are collected; the parameters are situated in the file MicroscopeConfiguration.ini.

Non relevant sections like [ScanCamera], [PreviewCamera], [PreviewAndBarcodeScanning], [Focus], [ReflectorTurret] and parameters, used for Pannoramic MIDI are left out. A short explanation of the parameters should help to understand the meaning of the parameter values.

 

Please make a security backup of the file MicroscopeConfiguration.ini before parameter values are modified!

 

 

[Version]

CurrentInifileVersion=1.9; actual MicroscopeConfiguration.ini file version since MScan.exe version 1.9 or higher, do not modify

[Microscope]

SerialNumber=2000;          a four digit number higher then 2000 defines the serial number of the SCAN-150, so the file MicroscopeConfiguration.ini is assigned to the hardware.

MicroscopeType=3DMic8;                        3DMic8 means SCAN -150 and Pannoramic SCAN

 

ScanCameraType=AVT Marlin F146C;  Camera type

 

PreviewCameraType=DFK21F04;          Preview camera type

 

BarcodeReaderType=MS904;                  for older scanners only (3DMic4), do not modify

 

 [HardwareLimits]                         The number of steps is defined without lost steps

ObjectGuideXMin=-800;                number of steps allowed in negative direction from Home1,2

ObjectGuideXMax=29900;                        number of steps allowed in positive direction from Home1,2

ObjectGuideYMin=-800;                number of steps allowed in negative direction from Home1,2

ObjectGuideYMax=73400;                        number of steps allowed in positive direction from Home1,2

FocusDeviceMin=-320;                 number of steps allowed in negative direction from Home1,2

FocusDeviceMax=3540;               number of steps allowed in positive direction from Home1,2

ReflectorTurretMin=-400;              number of steps allowed in negative direction from Home1,2

ReflectorTurretMax=58700;          number of steps allowed in positive direction from Home1,2

·        

·        

·        

StepSizeXinMicrometer=1;                                   do not modify

StepSizeYinMicrometer=1;                       do not modify

ScannableAreaPositionXMin=2200;      the specimen holder is even not touched by the focus pin

ScannableAreaPositionXMax=26000;   the medium wide slide should be touched by the half diameter of the focus pin, the step size for adjustment is 100 steps

ScannableAreaPositionYMin=22646;    starts usually with 22000 and is modified by the preview calibration program.

ScannableAreaPositionYMax=73400;   the specimen holder is not touched by the focus pin

ObjectGuideXYTimeDelayBeforeImageAcquisition=0;           A value between 0 and 30 ms can help to eliminate vibration after stage movements before the scan camera image is taken; 0 means no wait, 30ms is recommended.

 [SlideLoading]

DeafultPositionX=29600;              can be the value of “ObjectGuideXMax”-100 steps; the slide loader inner finger should not touch the slide clamp if the slide is inserted and the slide loader movable part is moving backward to the slide position of the magazine.

 

DeafultPositionY=18000;              the focus pin moves on the metal part of the specimen holder and the X-motor limits can be reached. Do not modify.

 

InsertSlidePositionX=700;                        the specimen holder position for slide insertion

 

InsertSlidePositionY1=400;          the specimen holder position for slide insertion at the beginning

 

InsertSlidePositionY2=600;          the final specimen holder position for slide insertion.

 

InsertSlidePositionY3=600;          the final specimen holder position for slide insertion.

 

SlideHolderReleasePositionY=8000;     the slide tightener is released, the slide clamp is spanned by the strong spring.

 

RemoveSlidePositionX1=-300;    the specimen holder position for slide removal at the beginning

 

RemoveSlidePositionX2=000;     the specimen holder position for slide removal at the 2. Phase

 

RemoveSlidePositionY=1100;     the specimen holder Y-position for slide removal

 

 [SlideLoader]

SlideLoaderTimeout=1200;                                              do not modify

 

SlideLoaderDelayTimeBeforeSensor=300;       If the outer sensor is attached it should be released after 300ms;  do not modify

 

 [MagazineLoader]

MagazineFirstSlidePosition=18;                         number of turns to reach the first slide position of the magazine, do not modify

 

MagazineRemovePosition=85;                           number of turns to reach the magazine remove position, do not modify

 

MagazineFirstSensorPosition=0;                                               do not modify

 

MagazineLastSensorPosition=85;                                 do not modify

 

StackerTimeout=10000;                                        the magazine load sensor should be attached in less then 10s, do not modify

 

StackerDelayTimeBeforeSensor=100;                          do not modify

 

StackerPreloadTime=2900;                                              do not modify

 

MagazineFeederMoveTimeAtPreload=0;                      do not modify

 

MagazineFeederTimeout=500;                           less then 500ms for one revolution is allowed, do not modify

 

MagazineFeederDeadTime=100;                                   do not modify

 

MagazineFeederDelayTimeBeforeSensor=100;         do not modify

 

MagazineFeederTryCount=10;                                       do not modify

 

MagazineFeederDelayTimeBeforeMagazineSensor=300;    after 300 ms check the left or right magazine sensor; do not modify

 

SlideCountPerMagazine=25;                              If SCAN-150=25, Pannoramic SCAN=25          If MIRAX SCAN=50; other values are not allowed