X-Y-stage unit;
SCS
For
technicians and partly for sales managers!
This description
handles the functionality and instructions to install and to check the
X-Y-Stage unit for the scanner Pannoramic Confocal.
Software relevant explanations are based on the software version 1.19.
· The unit is
derived from the scanner “P250” and the specimen holder is derived from the
PMIDI.
Contents
Functional
overview of the X-Y-Stage
Check the hysteresis
in Y-direction
Dismount or mount the
carriage drive unit
The X-Y-Stage unit is used to
move the specimen holder in X- and Y-direction for the slide load or unload action,
to reach positions on the slide (and so the tissue) while the slide scan
process and to secure the slide position.
The drive for the movements
is realized with stepper motors.
The X-Y-Stage unit is mounted
with a dovetail mounting, so the correct position is reached again
automatically after the unit was removed and mounted again; see also ”Exchange the X-Y-stage
unit”.
The allowed slide dimensions are:
Length: 75.00 to
Width: 25.00 to
Thickness: 00.95 to
In relation to the
X-Y-stage unit of the
Exchange of the X-Y-Stage unit is
necessary:
- If one of the stepper
motors is faulty.
- If the
maximal allowed hysteresis
of 4 steps in Y-direction is exceeded.
- If the shape
of any part is deformed or a part is broken.
- If the
X-Y-Stage unit has any fault
and you are unable to fix it.
- In any cases,
please refer first to the chapter “Before
you start to replace units”!
Requirements
- Service
program for slide scanners version 1.19 or higher (SlideScannerService.exe) with
actual license file.
- Slide scanner
and Slide scanner Viewer software (SlideScanner.exe,
SlideViewer.exe)
with dongle or actual license files.
- 1.5, 2.5, 3
and
- Hardware and construction knowledge
of the scanner PCON.
Attention: Do not mix the
versions of SlideScanner.exe and SlideScannerService.exe! Always use these programs with the same
version number. Otherwise the SlideScannerService.exe program could produce unwanted results and SlideScanner.exe does not work correctly or even freeze!
Since the software version 1.15
the units of the scanner are configured in the file
“MicroscopeConfiguration.ini”, section [Microscope].
The actual version of the X-Y-stage unit in the scanner Pannoramic
Confocal is “ObjectGuideXYZType=OGXYZ_FLASH4”.
· The
path of the file MicroscopeConfiguration.ini, in the software version with the
operating system Windows® 7 is:
C:\ProgramData\3DHISTECH\SlideScanner\MicroscopeConfiguration.ini
[Microscope]
SerialNumber=PCON_xxx
MicroscopeType=3DMic10
MicroscopeSubtype=Confocal
ScanCameraType=
PreviewCameraType=CVrmc_m8_pPro
BarcodeReaderType=PreviewCamera
LoaderType=SL_1Mag_12Slide_Sensor_Horizontal2
CameraChangerType=CC_none
ReflectorTurretType=RT_None
BrightfieldLightSourceType=RGBLedLight
ObjectiveChangerType=OC_2Pos
ObjectGuideXYZType=OGXYZ_FLASH4
FlashUnitType=NoFlashUnit
NDFilterType=NDType_None
PreviewLightType=PreviewLightUnitType_Type2
ShutterMotorType=Shutter_Motor
PowerSwitchBoardType=PowerSwitchBoard_Type1
ConfocalUnitType=ConfocalUnitType_Aurox
WaterFeederType=WaterFeeder_Type1
Remark
ObjectGuideXYZType=OGXYZ_1; not used today; the specimen holder
can hold slides with a thickness of 1.0mm nominal; the stepper motor contains
its control electronics (the same construction as in the PSCAN150).
ObjectGuideXYZType=OGXYZ_FLASH2; the Y-direction is 23 rotor revolutions
long, the specimen holder can hold slides with a thickness of 0.95 … 1.20mm,
the control of the stepper motor is realized in the “X-, Y-, Z-motor”
controller; a darkfield preview can not be created.
ObjectGuideXYZType=OGXYZ_FLASH3; this is the most recent version today; the
Y-direction is now 28 rotor revolutions long, it is prolonged by about 16mm (5
rotor revolutions) in relation to the previous version; the specimen holder can
hold slides with a thickness of 0.95 … 1.20mm (in P250 only!!); now a darkfield
preview can be created.
This value is used to define
the version P250 Flash!
ObjectGuideXYZType=OGXYZ_FLASH4; The firmware of the X- and Y-stepper motor
driver is modified to reach the scan speed of 130fps since the software version
1.17. Use this value only, if the firmware upgrade is done!
·
In the Pannoramic
Confocal only slides with a thickness of 0.95mm to 1.05mm may be inserted!
· For
more detailed information please refer to the chapter “Section [Microscope]”
The dovetail mounting ensures the proper position of the X-Y-stage unit
in relation to the tray loader unit and the focus unit.
The specimen holder
holds and secures the specimen (slide) during the scan process and allows an automatic
exchange of the slide.
Length: 75.00 to
Width: 25.00 to
Thickness: 00.95 to
See also: “Specimen holder”, “Principle of focusing” and
“Parallelogram”
The rotor drives the
transport nut via the spindle. The transport nut is mounted to the carriage
which is moving on the rails.
The X-carriage contains the entire Y-unit.
The Y-carriage moves the parallelogram with the specimen holder.
· The achieved
resolution in X- and Y-direction is: 1μm/rotor
step
· The guaranteed
movement range of the X-carriage is 28800 rotor steps (it means 28.8mm)
· The guaranteed
movement range of the Y-carriage is 89600 rotor steps (it means 89.6mm)
· The parts and
units of the carriages need neither maintenance nor mechanical adjustments.
Components
and construction
The X- and
Y-stepper motor is unexceptionally and always driven in micro stepping. This
way very precise movement is reached. One revolution of the motor axle is
divided into 3200 steps. The forward direction of the motors axle is counter
clock wise, CCW.
- The control
electronics of the X- and Y-motor is realized in the “X-, Y-, Z-motor”
controller.
- The parts and
units of the stepper motors need neither maintenance nor mechanical
adjustments.
See also: Types of stepper motors
“Stepper motors
without control electronics” mounted
The motor cable
headers are connected at the appropriate position to the control electronics
unit.
Important
Please do not use pliers to loosen or tighten the cable header lock
nuts. If there is too much force used on the connector headers, the soldering
of the connector may be destroyed or broken and the appropriate motor will not
work or may working very noisy.
|
Motor cable headers; working
connections |
||
|
Unit |
Connector |
Identifier |
|
Z-motor; female |
CF12 |
|
|
Z-motor; male |
CF13 |
|
|
Y-motor; female |
CF14 |
|
|
Y-motor; male |
CF15 |
|
|
X-motor; female |
CF16 |
|
|
X-motor; male |
CF17 |
|
|
Power for Aurox CC 88 |
|
|
· 
The length of the
cables is so dimensioned, that every motor connector pair may be reached with
any motor cable header pair.
Important
· For
test purposes and fault detection, the motor cable header pair may be connected
to another motor output; e.g. the X-motor headers are connected to the Y-motor
output.
· In
this example, the service program Y-direction tools will be used to move the
X-stage!!
· Before
SlideScanner.exe will be started, the motor cable headers have to be connected
to their original, correct motor connection!
See also: “X-Y-Z-motor control” and “Motor cable headers”
The carriages are used to move
the specimen holder and so the slide in the X-direction and the Y-direction.
The dovetail foot is mounted
onto the X-direction mounting plate from beneath and guarantees the proper
mounting and fixing of the entire X-Y-stage unit.
The static part of
the X-stage consists of the following parts:
- The X-direction mounting plate with the mounted
X-motor, the dovetail foot
(mounted from beneath), the leading rails, the upper and lower limiters.
The dynamic (moveable) part
of the X-stage consists of:
- X-stepper rotor with spindle and transport nut
- The transport nut is mounted to the Y-stage unit
mounting plate and drives so
- The entire
Y-stage unit.
The static part of
the Y-stage (the X-carriage) consists of the following parts:
- The Y-direction mounting plate with the mounted
Y-motor, the leading rails, the upper and lower limiters.
The dynamic (moveable) part of
the Y-stage consists of:
- The Y-stepper rotor with spindle and transport nut
- The transport nut is mounted to the Y-carriage and
this drives so
- The parallelogram with
the specimen
holder.
The carriages are mounted and
leaded with 2 rails for each direction.
The X and Y rails ensures a
slippage free movement of the carriages in X- and Y- direction. The X-carriage
contains the entire Y-part. If the motor starts rotating, the spindle drives
the carriage in the direction, defined by the rotating direction of the rotor.
The mechanical dimensioning of the X-Y-stage allows reaching nearly each part
of the slide by the objective, except the barcode area (restrictions are given
by the slide holding mechanics of the specimen holder;
see also the scan area).
- The carriages can be moved independently from
each other in any direction inside the appropriate hardware limits. Take
into account, that the slide stud area must not be touched neither with
the objective nor the focus pin!
The X- and
Y-spindle respectively are connected with a pressure mounting directly to the stepper
motor; on the thread
of the spindle the transport nut is situated and the transport nuts are
mounted onto the X- respective Y-carriage.
The construction
of the carriage transport
nuts ensures a nearly slippage free movement of the carriages, a maximal slip of 4 μm
(=4 motor steps) is allowed. The slippage of the transport nut is minimized by
the use of the counter nut and the compression spring.
The use of the stepper motors
micro stepping mode, combined with the accuracy of the mechanics allows
achieving a resolution of 1μm longitudinal movement per rotor step.
The length of the
movement of each carriage is limited by two limiters, one for the upper and one
for the lower limit, separately for the X- and Y-direction respectively. This
way, the mechanical construction gets a start and an end position.
If the mechanical limiter is
reached by the appropriate carriage, the movement stops and, if further steps
have to go, these steps are lost. This behavior creates “lost steps”.
During the detection of the hardware limits:
Creation of lost steps is
used to find and determine the hardware upper and lower limit. The first number
of steps that do not create lost steps is used as hardware limit; the accuracy
is 100 steps (0.1mm).
Example:
· If we found, that
the step number of 1200 steps in negative direction after Home1,2 does create lost steps more than +-2
steps and
· The step number of
1100 steps in negative direction after Home1,2 does not create lost steps (not more than +-2 steps) the negative hardware limit will be -1100
steps.
If we are defining the upper
limit, the same principle is used; first we creating lost steps then we
decreasing the number of steps to go by 100 steps until no steps are lost
during the movement.
During all other actions:
Lost motor steps are unwanted
during slide insert and removal actions and the sample scanning process,
because the counting and reporting of steps mismatches the real number of steps
gone. Therefore, the limits are defined by using the last possible number of
steps without lost steps and an accuracy of 100 steps (=0.1mm) see also later
“Setup procedures”.
The home position does not
define the mechanical limit. Either in -X- and either in -Y-direction there are
several hundred more steps possible. The theoretical, absolute limits are
defined as shown in the figures “X-direction” and “Y-direction” respectively.
· The unit is faulty
(or incorrectly adjusted), if there are more than 1600 steps possible in negative
direction without jamming, counted from Home1,2!
· The unit is faulty
(or incorrectly adjusted), if there are more than 1600 steps possible in
positive direction without jamming after 28800 steps in +X-direction or 89600
steps in +Y-direction respectively!
The unit should also fulfill the following
requirement:
· The negative limit
“Y-min” < -500 steps.
See also: “How to define the hardware
limits”
The following
adjustments are done only, if the motor has to be exchanged, the X-Y-carriage
drive unit was removed or the hysteresis in X- or Y-direction is too much.
The resolution of the stepper motor by 3200 steps/revolution and
the construction of the spindle together with the transport nut allow a
resolution of 1μm longitudinal movement per rotor step; the counter nut
with spring reduces the slippage (resulting in hysteresis) if the rotation
direction changes.
The only difference between the Y-carriage drive unit and the X-
carriage drive unit is the length of the transport spindle; the spindle of the
X-unit is some centimeter shorter.
Precautions
- In all procedures, named below, handle the specimen holder and the parallelogram
with special care; the weight of the X-Y-stage unit should never be
carried by the specimen holder or the parallelogram at all. Not even
partially!
- Do not remove the mounting of the specimen holder or the
parallelogram; do not manipulate them in any form!
For more information, please refer also to: P250_Y_drive_unit_exchange
The spindle is mounted
to the motor axle by using a compression mounting; this guarantees also a
slippage free connection and the spindle can be dismounted from the motor axle
easily.
This mounting construction guarantees also a centered mounting of the
spindle.
· The pressure nut
can be rotated with a small head 8mm open end wrench, the pressed part can be
hold with a small head 6mm open end wrench!
· 
Loosen and remove
the pressure nut and pull the pressed part from the rotor axle.
The claws of the pressed part are fitting the diameter of the rotor
axle; the spindle is glued into the pressed part.
Assemble
the connection
· 
Put the pressure nut
in the right direction onto the motor axle.
· Fit the claws onto
the rotor axle.
· Drive the pressure
nut onto the pressed part manually, until it stops.
· Push the pressed
part on the rotor axle against the motor housing until it stops.
· Hold the pressed
part with the 6mm wrench and drive the pressure nut with the 8mm wrench.
· The connection is
correct, if the pressed part is hold on the rotor axle and the gap between
pressure nut and motor housing is not more then 1mm.
· 
The gap between
the pressure nut and the motor mounting must not exceed 1mm!
Transport
spindle; Multi (4) thread
spindle
The spindle
(together with the transport nut) is used to transform rotation into slippage free
longitudinal movements. The four threads on the spindle guarantee a precise
movement, increase the torque of the mechanical drive and help to reduce or
eliminate slippage and hysteresis.
Rotor axle slip
Another important source
of slippage can be the rotor bearing of the motor. Because the rotor has
longitudinal load also, the position of the ball bearings of the rotor must not
change if the rotation direction is changed.
· The rotor axle
must not have slippage.
To check the slippage of the motor axle manually, use a force of about 1
N. Longitudinal movements of the rotor axle must be impossible. Take into
account, that a slip of
Eliminate the slippage of the rotor axle
Originally, the rotor
bearing has a spring to eliminate the slip, but the force of the original
spring is often not enough; even if the motor axle has a longitudinal load like
in the X- or Y-carriage. Therefore the original solution was exchanged by using
a flat and a curved washer; the curved washer acts as a spring.
If both washers are inserted well, the slip has to be eliminated.
1.
Remove the cover
mounting bolts of the motor (1).
2.
Pull the back cover carefully backward and put it onto
the table as shown (2).
3.
Insert the flat washer first; then the curved washer
so, that its perimeter contacts the rotor bearing as shown (3).
4.
Fit the back cover to the motor and take care of the
washers, they should not falling out.
5.
Drive in the mounting bolts of the back cover; check
the easily movement and the fitting of the rotor, then tighten the mounting
bolts and check the easily movement of the rotor again by hand.
6.
Check the correct movement of the motor with the
service program and listen the sound also; rotate the motor by more ten
revolutions forward and backward some times.
7.
If there can be a sliding or sanding sound listened or
the motor moves strong, loosen the mounting bolts again a little bit and fit
the rotor mounting more precise.
Transport-
and counter nut with spring
The transport nut
transforms the rotation of the spindle into a longitudinal movement of the connected
peripheral; it moves the X- or Y-carriage to the desired position.
The thread of the spindle and the nut moves the peripheral by
To reach the appropriate limits of the mechanical drive, the position of
the transport nut on the spindle is important; the position can be defined with
an accuracy of a ¼ revolution (because there are 4 threads), it means
800 motor steps.
The limits have to be less than 1600 steps in negative direction counted
from Home1,2 and less than 1600 steps after 28 revolutions of the spindle (if
Y-carriage); see also “Hardware
limits” and “Find the
hardware limits for the Y-carriage”; both limits have to be fulfilled if
the adjustment is finished; otherwise dismount the carriage
drive unit, define the position of the transport nut in relation to the
spindle with +- ¼ full turn, mount the carriage
drive unit and check the limits again.
To eliminate the
slip of the mechanical drive (to reduce and eliminate the hysteresis), the spring
forces the transport nut away from the counter nut. The force of the spring
guarantees the appropriate pressure of the transport nut against the threads of
the spindle. By using the appropriate start point of the threads, the counter
nut can be positioned in relation to the transport nut in the same way as the
transport nut; the position can be defined by a ¼ revolution (because
there are 4 threads), it means 800 motor steps. The force of the spring and the
position of the counter nut are correct, if the counter nut is less than or
maximal 0.5 turn in distance from the transport nut with fully compressed
spring and the slip (checked after
assembly; with the SlideScanner program) is less than or equal to 4 motor
steps for the Y-carriage; or less than or equal to 8 motor steps for the
X-carriage when the rotation direction of the spindle had changed.
If the spring can not act, (the counter nut is pressing the transport
nut without a spring acting distance) the drive does not move or is moving too
strong. See also “To check the
maximal hysteresis in Y-direction” and “Optics and illumination”, “Stitching”. If the
hysteresis is too much, reduce it by reducing the distance between transport
nut and counter nut; check also the slip of the motor axle and other drive
connections, see
above.
See also: P250_Y_drive_unit_exchange
Important
The parallelogram
should not be adjusted in the field, there are no adjustable parts. The
mounting bolts of the parallelogram and the X-Y-plane adjustment bolt are
adjusted. Please do not screw them!
·
Necessary adjustment tools are often not available in
the field.
The parallelogram allows the
shifting of the X-Y-plane in Z-direction for focusing the FOV without rotating
the X-Y-plane. On one side of the parallelogram the Y-carriage is mounted; on the
other side the parallelogram holds the specimen holder.
The limiter is constant 0.8mm
and so the maximal movement of the parallelogram is limited.
·
The
entire parallelogram and the specimen holder are very sensitive components, because these guarantee the X-Y-plane in
relation to the objective.
If
the parallelogram should be exchanged in the field; please refer first to:
“How to adjust the
parallelogram”
The parallelogram
is not a separate changeable spare part, therefore, the X-Y-Stage unit must be
changed if there is an irresolvable fault on the parallelogram.
- The movement of the parallelogram is limited to
be 0.8mm in the PCON.
See also: Adjust the
parallelogram
This specimen
holder is designed to hold the slide, to secure it during scan operation and
allows an automatic slide exchange operation. To loosen the slide during insert
or remove procedure, the slide tightener opens the slide stud.
In its released state the
“Assembled arm” is forced by the spring (not shown) to hold the slide via the
studs. If the slide will be inserted or removed, the Y-motor goes to slide
insert or remove position and the X-stepper goes forward nearly to its positive
limit and contracts the spring in “push” direction via an arm mounted beneath
of the tray holder (the release plate), to release the slide. The longest
allowed slide (76,00mm) will be inserted nearly until the bumper edge.
Length: 75.00 to 76.00mm
Width: 25.00 to
26.00mm
Thickness: 00.95 to 01.05mm
See also: “Physical
construction of the specimen holder”
If the Y-stage is
in Home1,2 position, or only some 100 steps away from it and the X-Y-stage moves
to the right (-X-direction), the slide tightener contacts the release plate on
the left side, the slide tightener moves to the left and the assembled arm will
release the slide.
·
If the X-stage moves in the negative direction the
slide tightener will contact the release plate from beneath and so, the slide
stud of the assembled arm will release the slide; a slide insert or removal
action can be performed.
·
During scanning, the slide tightener will never touch
the release plate; the slide is hold and secured in the slide studs.
Slide holding and scan
area; PCON
The
slide is held in the specimen holder with the help of three slide studs,
situated on the longer edges as shown.
The surrounding of the slide must be defined in “MicroscopeConfiguration.ini”
section [HardwareLimits] to avoid collision of the focus pin or the objective
with the slide stud.
·
Never touch the slide stud with the
objective or the focus pin!
See also: “Areas
of the slide”, “Physical
construction of specimen holder” and “Define the scan area”
Adjustments
for the X-Y-carriage unit
The following procedures are
described for the Pannoramic 250 and based on the service program version 1.15.
Find the hardware
limits for the X-carriage
This procedure must
be done only if the scanner unit or the X-Y-stage was changed.
This procedure has to be done
if the drive unit for the X-direction was exchanged.
· Insert a medium large slide
and set the focus motor to -300 steps.
Find the negative limit in -X-direction
1.
With the service program set the Y-carriage to
Home1,2.
2.
Set the X-carriage to Home1,2.
3.
With the service program go forward to the X-motor
position -1200 steps.
4.
Go backward +1200 steps.
5.
Press Home1 (only). There should be not more than +-2
steps difference to Home1. If there are more steps lost, decrease the actual
absolute number of steps by 100 and repeat from step
6.
If there are not more than 2 steps difference to
Home1, increase the number of steps by 100 and repeat from step
7.
The negative limit is found correctly if the motor
movement has no steps lost and the actual absolute number of steps, increased
by 100 would produce lost steps. The found negative limit can differ by more
100 steps from unit to unit. The reason is the tolerance of the components and
the found thread starting position of the spindle in relation to the transport
nut.
8.
Update the value of the parameter “ObjectGuideXMin” in the file “MicroscopeConfiguration.ini” section [HardwareLimits] with the found
number of the actual steps and save the file.
Find the positive limit in +X-direction
9.
With the service
program set the X-carriage unit to Home1,2.
10.
Go forward to the X-motor position +29700 steps.
11.
Go backward 29700 steps.
12.
Press Home1 (only). There should be not more than +-2 steps
difference to Home1. If there are more steps lost, decrease the actual number
of steps by 100 and repeat from step
13.
If there are not more than 2 steps difference to
Home1, increase the number of steps by 100 and repeat from step
14.
The positive limit is found correctly if the motor
movement has no steps lost (max. 2 steps) and the actual number of steps, increased
by 100 would produce lost steps. The found positive limit can differ by more
100 steps from unit to unit. The reason is the tolerance of the components and
the found thread starting position of the spindle in relation to the transport
nut.
15.
Update the value of the parameter “ObjectGuideXMax” in
the file “MicroscopeConfiguration.ini” section [HardwareLimits] with the found
value and save the file.
16.
Check the found limits by using the number of steps,
used as parameter value in the file “MicroscopeConfiguration.ini” section
[HardwareLimits]. Lost steps have not to occur.
Find the hardware limits for the Y-carriage
This procedure
must be done only if the scanner unit or the X-Y-stage was changed.
This procedure has to be done
if the drive unit for the Y-direction was exchanged.
·
Move the X-carriage +3200 steps from Home1,2; hereby take care on the focus pin and avoid
collision with the slide studs or the specimen holder!
Find the negative limit in -Y-direction
Repeat the steps,
described in the procedure above “To find the negative limit in -X-direction”
logically with the Y-carriage.
·
Update the value of the parameter “ObjectGuideYMin” in
the file “MicroscopeConfiguration.ini” section [HardwareLimits] with the found
number of the actual steps and save the file.
Find the positive limit in +Y-direction
Repeat the steps,
described in the procedure above “To find the positive limit in +X-direction”
logically with the Y-carriage. The positive limit is found correctly in the
near of 90 000 steps or even before the specimen holder will be touched.
·
Update the value of the parameter “ObjectGuideYMax” in the file “MicroscopeConfiguration.ini” section [HardwareLimits] with the found
value and save the file.
Check
the hysteresis in Y-direction
General
The software
divides the sample to be scanned, seen by the preview camera into fields of
views; the size of the FOV depends on the resolution of the scan camera and the
magnification of the camera adapter. Each field of view contains a small part
of the neighbor FOV. In this way, stitching becomes possible. Because the
capturing of the FOV’s is done on a meandering course, the Y-direction is often
changed. If the hysteresis in Y-direction is too much, stitching will not work
correctly; therefore, we have to check the hysteresis in Y-direction. The
maximal allowed hysteresis is 4 μm (=4 motor steps). We comment that this
hysteresis decreases itself by some motor steps after some sample scan
procedures, even if the X-Y-stage is brand new.
Because the X-direction is
never changed during a sample scan process (the direction is always +X), the
X-hysteresis is not critical and can be some steps more (max: 8 steps).
Check the maximal hysteresis in Y-direction
·
Start the program
“SlideScanner.exe” with the service password.
·
In the tab “Focus” produce a sharp life view; adjust
the exposure time and the white balance as required.
·
In the tab “Service” select “Microscope control”.
·
Select the option “Cross line on image”.
·
Set a zoom value of about 2,73.
·
In the part of the X-Y-control select a step size of
two steps and go upward (or downward), until the tissue moves.
·
Now go in opposite direction and count the clicks
until the tissue moves. If more than 3 clicks (6 steps) are required, the
hysteresis in Y-direction is too much.
·
If the stage is moved to the left or to the right
respectively, the hysteresis in X-direction can be determined.
Dismount or mount the carriage drive unit
Dismount the carriage drive unit
·
Remove
the transport nut mounting bolts from the appropriate carriage (X- or
Y-Mounting).
· Remove the motor
mounting bolts.
·
Pull
the motor together with the entire carriage transport unit out of the carriage.
See also: How to exchange the
P250_Y_drive_unit
1.
Set the motor to Home1,2.
2.
Screw the transport nut onto the spindle some revolutions;
the motor stays always in Home1,2 except explicitly specified otherwise.
3.
Insert the carriage drive unit (do not drive in the
bolts!) and check the position of the transport nut in relation to the bolt
drillings on the carriage; the drillings of the carriage should fit the
transport nut near to the negative limit (less than 1.5mm).
4.
Remove the transport unit and adjust the position of
the transport nut in relation to the carriage mounting more precise by using
half and quarter revolutions also.
5.
Set the motor to Home1,2.
6.
Insert the carriage transport unit, drive in the motor
mounting bolts and the transport nut mounting bolts and check the negative
limit; see also above “Limiters”
and adjustment procedures “To find the negative limit”. The negative limit is
often found in the near of -800 steps (but this is not a requirement).
7.
If the negative limit does not fit the requirements
(more than 1600 steps are possible in negative direction) remove the carriage
drive unit again and adjust the position of the transport nut in relation to
the spindle by using the next or the previous start point of the thread; this
way the limit can be adjusted with an accuracy of 800 steps (a ¼
revolution) and this fulfill the requirements always (this is done only, if the
adjustment by using half turns does not deliver the successful result).
8.
Repeat from step 4 until the requirements of the
negative limit are fulfilled.
9.
Check the positive limit; see also “Define hardware limits”.
10.
By loosening the motor mounting bolts and tighten them
in the correct motor position, the straightness of the drive unit can be found
and adjusted.
Remove or mount the X-Y-stage unit
The X-Y-stage may be inserted
or removed only, if the focus unit is not mounted!
- Therefore, the focus unit has to be removed
first.
Focus unit
mounting
The mounting of the
focus unit with objective changer is realized with dovetails; these are hold by
dovetail fixing clamps.
- Rotate the 2.5 hex key wrench
clockwise to open (loosen) the dovetail clamp.
Remove the X-Y-stage unit
1.
Move the X-direction to
Home1,2 and move the Y-direction to Home1,2.
2.
Then move the X-direction to X=+28 000 steps.
3.
Move the Y-direction to Y=+80 000 steps.
5.
Disconnect the cables, loosen the X-Y-stage unit
fixing bolt and remove the X-Y-stage unit by pulling it backward.
X-Y-stage unit mounting
The dovetail
clamp’s fixing bolt is driven in opposite direction in relation to the focus
unit; see on the right.
Mount the X-Y-stage unit
6.
Move the X-stage to the position 28000 steps.
7.
Insert the X-Y-stage unit until it stops and tighten
the fixing bolt.
9.
Connect the cables of the focus unit, the Y-part
cables and the X-part cables.
10.
Check the position of the slide tightener; it should
release the slide clamp if the Y-direction Homr1,2 and the X-direction Home 1,2
is found.