The P250 includes all possibilities and features like different camera selection for fluorescent scan sessions and brightfield scan sessions, selection of the objective 20x or 40x magnification for brightfield and fluorescent scan sessions likewise; it is able to handle slides until a thickness of 1.20mm.

 

The allowed slide dimensions are defined as

 

Length:               75.00 to 76.00 mm

Width:                 25.00 to 26.00 mm

Thickness:       00.95 to 01.20 mm

 

·       Since 2011 on the market

·       The slides are hold in magazines with 25 slide positions

·       9 magazines can be hold in the input / output stack and 1 magazine in the feeder channel, so the amount of slides in 10 magazines can be scanned during 1 session.

·       The specimen holder is modified (in relation to the SCAN150); so slides with a thickness of 1.20mm can be inserted.

·       Flashlight BF illumination and the use of higher speed cameras allows higher scan speeds.

·       Designed for brightfield an fluorescent scan sessions.

·       Exchange of the objective 20x or 40x magnification; software controlled between 2 slide scan sessions.

·       Exchange of the camera, software controlled, between BF and FL slide scan sessions.

 

The P250 exists in a full version (P250_FL) where the Reflector turret unit is included and a more cost-effective version (P250_BF) without the Reflector turret unit, so only brightfield scan sessions are possible.

 

 

       Pannoramic 250

 

 

 

 

 

 

 

                   Magazine, used in the P250

·       A gear on the rear of the magazine allows the movement of the magazine in the feeder channel.

·       The permanent magnet is used to detect the presence of a magazine in the input stack and the state “Output stack full” in the output stack.

·       By filling magazines in the input stack and continuously removing finished magazines from the output stack during the scan session is in progress, a “Continuous mode” of the scan session can be performed.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

                   Variants of the scanner

 

The following differentiation is not done in the description; all variants are included. References to the appropriate variant can be found at the appropriate place, usually in the chapter “configure” of the unit or in the section [Microscope] of the file “MicroscopeConfiguration.ini”.

 

 

 

P250

 

The P250 is the base version of the scanner; it contains the objective changer as well as the motorized camera changer, but the X-Y-stage is the same as used in the SCAN.

 

Restrictions

·       Darkfield preview is impossible

·       Brightfield scan procedure can not be executed with the 40x magnification

·       True, until the software version 1.15

 

Values in the section [Microscope]

 

BrightfieldLightSourceType=FlashLight2010

ObjectGuideXYZType=OGXYZ_2;         

FlashUnitType=FlashUnit_Type2

PreviewLightType=PreviewLightUnitType_Type1

 

 

 

P250 Flash

 

The P250 Flash is an enhanced version of the scanner; it contains improvements concerning preview creation and possible scan magnification.

The X-Y-stage as well as the flash light illumination is modified in relation to the previous version.

 

Improvements

·       Darkfield preview is possible

·       Brightfield scan procedure can be executed with the 40x magnification; depending on the value of  the parameter “BrightfieldLightSourceType”

·       Maximal scan speed is 30 fps

·       True, until the software version 1.16;     see also:        Upgrade to SW version 1.16

 

 

Values in the section [Microscope] are

 

BrightfieldLightSourceType=FlashLight2010; Brightfield scan procedure possible with 20x magnification only

BrightfieldLightSourceType=FlashLight2012; Brightfield scan procedure possible with 20x or 40x magnification likewise

ObjectGuideXYZType=OGXYZ_FLASH3;     

NDFilterType=NDType2

FlashUnitType=FlashUnit_Type2

PreviewLightType=PreviewLightUnitType_Type2

 

 

 

 

P250 Flash 2

 

The enhancement increases the scan speed drastically in opposite to the previous versions.

 

Improvements

·       Maximal scan speed is 130 fps

·       True, since the software version 1.17;   see also:        Upgrade to SW version 1.17

 

Values in the section [Microscope] are

 

BrightfieldLightSourceType=FlashLight2010; Brightfield scan procedure possible with 20x magnification only

BrightfieldLightSourceType=FlashLight2012; Brightfield scan procedure possible with 20x or 40x magnification likewise

ObjectGuideXYZType=OGXYZ_FLASH4;      This modification includes, that all hardware enhancements are executed and the speed of 130fps can be reached

NDFilterType=NDType3

FlashUnitType=FlashUnit_Type2

PreviewLightType=PreviewLightUnitType_Type2

 

 

Required upgrades

 

To reach the scan speed of 130fps or to modify a P250 FLASH into the version P250 FLASH2, the following modifications are required:

·       X-and Y-stepper motor driver firmware upgrade

·       Exchange the brightfield scan camera

·       Exchange the PC

·       Implement the Matrox Radient board

·       Install the software version 1.17 or higher

 

Remark

If the required upgrades are not or only partly executed, the software version 1.17 can be installed as usually, but the maximal scan speed of 130fps can not be reached.

 

·       In this case, please use the following settings:

 

Values in the section [Microscope] are

 

BrightfieldLightSourceType=FlashLight2010;             Brightfield scan procedure possible with 20x magnification only

BrightfieldLightSourceType=FlashLight2012;         Brightfield scan procedure possible with 20x or 40x magnification likewise

ObjectGuideXYZType=OGXYZ_FLASH3;     

NDFilterType=NDType2

FlashUnitType=FlashUnit_Type2

PreviewLightType=PreviewLightUnitType_Type2